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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Peptidoglycan Synthesis01:28

Peptidoglycan Synthesis

Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan biosynthesis begins in...
Carbon-13 (¹³C) NMR: Overview01:10

Carbon-13 (¹³C) NMR: Overview

Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
Special Staining Techniques01:13

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
Protein Folding01:22

Protein Folding

Overview

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関連する実験動画

Updated: Jun 23, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

Staphylococcus aureus ペプチドーグリカンは,炭素13のスピン拡散による三次構造である.

Shasad Sharif1, Manmilan Singh, Sung Joon Kim

  • 1Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.

Journal of the American Chemical Society
|May 8, 2009
PubMed
まとめ

研究者は,Staphylococcus aureusの細胞壁を研究し,ペンタグリサイルクロスリンクが,驚くほどグリカン鎖に近いことを明らかにしました. この発見は,ペプチドグリカン格子構造のモデルを情報化する.

科学分野:

  • 微生物学 微生物学とは
  • バイオケミストリー バイオケミストリー
  • 構造生物学 構造生物学とは

背景:

  • 複合ポリマーであるStaphylococcus aureusの細胞壁ペプチドグリカンの三次構造は,ほとんど不明のままである.
  • この構造を理解することは,標的型抗菌剤戦略を開発する上で極めて重要です.

研究 の 目的:

  • Staphylococcus aureus peptidoglycanの三次構造を部分的に特徴付けるために.
  • 主要なポリマー連鎖へのクロスリンクセグメントの空間的近接を調査する.

主な方法:

  • S. aureusの細胞壁におけるペンタグリサイルクロスリンクセグメントの利用された炭素13 (13C) のラベル付け.
  • 専門の核磁気共鳴 (NMR) 技術,中帯のみの交換検出 (CODEX) を採用し,スピン拡散を測定した.
  • [1-13C]グリシンで濃縮された媒介で培養されたS. aureusから分離された細胞壁.

主要な成果:

  • スピン拡散測定は,ペンタグリシルブリッジが隣接するペプチドグリカン重複ユニットのグリカン鎖から5 Å以内であることを示した.
  • この近接性は,ペプチドグリカン成分の空間的配置に関する以前の仮定に異議を唱える.

さらに関連する動画

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

関連する実験動画

Last Updated: Jun 23, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

結論:

  • この研究では,ペプチドグリカン網のモデルを提案し,ペプチド茎は,グリカンメインチェーンの垂直平面で互いに平行に方向づけられています.
  • この洗練された構造モデルは,Staphylococcus aureusの細胞壁構造に関する新しい洞察を提供します.