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化学的に標識されたヌクレオチドとオリゴヌクレオチドは,ナノ孔で感知するためのDNAをコードする

Vinciane Borsenberger¹, Nick Mitchell, Stefan Howorka

¹Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London WC1H OAJ, UK.

Journal of the American Chemical Society

|May 16, 2009

まとめ

DNA鎖の化学タグは,電気ナノ孔記録のための配列情報をコードする. この方法はDNA変異を区別し,固体ナノポールの新しい検出戦略を提供します.

科学分野:

分子生物学は分子生物学である.
ナノテクノロジーナノテクノロジー
バイオフィジックス生物物理学

背景:

レポーターグループによる核酸およびオリゴヌクレオチドのラベル付けは,配列特異のDNAセンシングに不可欠です.
光タグは一般的な例ですが,固体ナノ孔には代替方法が必要です.

研究の目的:

化学タグが電気ナノ孔記録のためのシーケンス情報をエンコードできることを実証するために.
毛孔工学とは無関係な新しいDNA検出戦略を開発する.

主な方法:

化学タグが付いた個々のDNA鎖は,電泳法を用いてナノスケールの毛穴を通過させられた.
毛穴を通過するタグされたDNA鎖の電気シグネチャが記録されました.
この方法は,薬剤耐性を与えるDNA点変異を区別することによって検証されました.

主要な成果:

化学タグは,DNA転位中のイオン電流ブロックで特徴的な電気シグネチャを生成した.
シーケンス情報は,これらの電気的シグネチャを通して,成功裏にエンコードされ,読み取られました.
この戦略は,薬剤耐性を与える変異を持つDNA配列を効果的に区別しました.

結論:

化学タグは,電気ナノ孔記録における配列情報をエンコードするための実行可能な方法を提供します.
このアプローチは,孔の修正を必要とせずに,固体ナノ孔の感知能力を高めます.
この戦略は,DNA配列分析と突然変異検出のための汎用的なツールを提供します.

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