クジラ種におけるポリモルフな単純な配列ロキーの保存
C Schlötterer1, B Amos, D Tautz
1Institut für Genetik und Mikrobiologie, Universität München, Germany.
Nature
|November 7, 1991
まとめ
パイロットクジラのために開発されたシンプルシーケンスの長さのポリモルフィズム (SSLP) プライマーは,様々なクジラ種でDNAを成功裏に増幅しました. 非コーディングDNA配列のこの高い保全は,クジラ間の遺伝子研究を簡素化します.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- 海洋哺乳類科学とは
背景:
- シンプルシーケンスの長さのポリモルフィズム (SSLP) は,真核DNAに共通しており,集団遺伝学にとって価値があります.
- パイロットクジラ (Globicephala melas) のSSLPは,以前,その交配システムと集団構造についての洞察をもたらしました.
研究 の 目的:
- パイロットクジラSSLPのために設計されたポリメラーゼ連鎖反応 (PCR) プライマーの種間適用性を調査する.
- 多種多様なクジラ種における単純な配列のロキにおける側面配列の保存を評価する.
主な方法:
- グロービセファラメラスのために開発されたPCRプライマーを使用して,様々なクジラの種で同類のロキーを増幅しました.
- 11種類のクジラ (歯のあるクジラとバレーンクジラ) からクローン・シーケンスされた増幅ロキーをクローン・シーケンスした.
主要な成果:
- ホモログのロキは,歯のあるクジラ (Odontoceti) とバレーンのクジラ (Mysticeti) の幅広い範囲で成功裏に増幅されました.
- シンプルシーケンス線を横切ったシーケンスには,極めて高い保全が認められ,推定3500万~4000万年にわたって平均差異はわずか3.2%であった.
- この低い分岐率は,現代のクジラ種の進化年齢を再評価する可能性を示唆しています.
結論:
- 1つのクジラ種のSSLPのために設計されたPCRプライマーは,保存された側面配列のため,他のクジラ種にも広く適用できます.
- この高い保全は,SSLPのDNA指紋を様々なケータイの遺伝子分析に使用することを容易にする.
- この発見は,クジラの種間の非コーディングDNAの異変率が著しく低いことを強調し,さらなる進化的研究を促した.
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