複数のアセンブリチャペロンは,プロテアソームの調節粒子ベースのバイオゲネシスを支配する
Minoru Funakoshi1, Robert J Tomko, Hideki Kobayashi
1Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520-8114, USA.
Cell
|May 19, 2009
まとめ
4つの組み立て因子,Nas2,HSM3,Nas6およびRpn14は,酵母19Sプロテアソーム調節粒子 (RP) ベースバイオゲネシスに不可欠であり,適切な26Sプロテアソーム形成を確実にするための重なり合っているチャペロンとして作用します.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- 26Sプロテアゾームは,真核生物の中央プロテアゼであり,20Sのコア粒子 (CP) と19Sの調節粒子 (RP) を含む.
- RPはカバーとベースサブコンプレックスで構成されていますが,その組み立て経路は十分に理解されていません.
研究 の 目的:
- イースト 19S プロテアソーム 調節粒子 (RP) ベースのバイオゲネシスを支配するアセンブリファクターを調査する.
- RPアセンブリと26Sプロテアソーム形成における特定のタンパク質の役割を解明する.
主な方法:
- インビヴォおよびインビトロ生化学測定法.
- 組み立て因子のための特定の遺伝子削除を持つ酵母菌株の分析.
- 複合体の形成とタンパク質の相互作用に関する研究.
主要な成果:
- 4つの保存されたアセンブリ因子 (Nas2,Hsm3,Nas6,Rpn14) は,酵母RP塩基生物生成に重要なものとして特定されました.
- Nas2は,Rpt4およびRpt5アテパゼと複合体を形成し,26Sプロテアソーム形成を促進する.
- Hsm3,Nas6,Rpn14は,アセンブリチャペロンとして機能し,ベースアセンブリと安定性に寄与します.
結論:
- プロテアソマのRPバイオゲネシスは,複数の,機能的に重なり合うチャペロンを必要とします.
- RPベースを形成する前に,サブユニットが特定のサブコンプレックスに組み立てられるモデルが提案されています.
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