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関連する概念動画

Mutations01:35

Mutations

31.2K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
31.2K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

4.0K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
4.0K
Absorption of Radiation01:05

Absorption of Radiation

1.6K
The rate of heat transfer by emitted radiation is described by the Stefan-Boltzmann law of radiation:
1.6K
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

5.6K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
5.6K
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

1.6K
Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
1.6K

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関連する実験動画

Updated: May 2, 2026

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
11:26

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light

Published on: September 12, 2014

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代替スプライシングに紫外線を照らす.

Matthew S Marengo1, Mariano A Garcia-Blanco

  • 1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA. matt.marengo@duke.edu

Cell
|May 20, 2009
PubMed
まとめ

DNAの損傷は,前メッセンジャーRNA (前mRNA) スプライシングの変化を誘発する. 新しい研究では,紫外線照射がRNAポリメラーゼIIハイパーフォスフォリレーションとより遅い転写を経由して代替のmRNA前スプライシングを活性化することを明らかにしています.

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは

背景:

  • DNA損傷に対する細胞の反応には,前伝達 RNA (pre-mRNA) スプライシングを含む遺伝子発現の調節が含まれます.
  • 代替スプライシングは最終的なメッセンジャーRNA (mRNA) 変種を決定し,アポトーシスなどの細胞運命を影響する.
  • プレ-mRNAスプライシングの調節は,転写と密接に関連しています.

研究 の 目的:

  • 細胞がDNA損傷後のプレ-mRNAスプライシングを変化させるメカニズムを調査する.
  • 紫外線 (UV) 照射後の代替スプライシングを調節する特定の分子イベントを特定する.

主な方法:

  • 紫外線曝露後のRNAポリメラーゼIIC端末領域 (CTD) のリン酸化状態の分析.
  • 紫外線照射への反応として転写延長率の測定.
  • 紫外線で処理された細胞における代替的なプレ-mRNAスプライシングパターンの評価.

主要な成果:

  • 紫外線照射は,RNAポリメラーゼII CTD.のハイパーフォスフォリレーションにつながります.
  • 転写延長率は,UV照射後に減少しています.
  • RNAポリメラーゼII活性におけるこれらの変化は,特定の代替スプライシングイベントの活性化と相関しています.

さらに関連する動画

The Lambda Select cII Mutation Detection System
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The Lambda Select cII Mutation Detection System

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Triplet Fusion Upconversion Nanocapsule Synthesis
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Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

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関連する実験動画

Last Updated: May 2, 2026

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
11:26

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light

Published on: September 12, 2014

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The Lambda Select cII Mutation Detection System
07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

7.5K
Triplet Fusion Upconversion Nanocapsule Synthesis
08:36

Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

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結論:

  • コトランスクリプションメカニズムは,DNA損傷,RNAポリメラーゼII改変,および代替スプライシングを結びつける.
  • RNAポリメラーゼIICTDのハイパーフォスフォリレーションとトランスクリプション延長の減少は,UV後の代替スプライシングの重要な調節因子です.
  • この経路は,DNA損傷に対する細胞の抗またはプロアポプト性反応に役割を果たします.