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関連する概念動画

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro08:27

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

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Here, a nanobar-supported lipid bilayer system is developed to provide a synthetic membrane with a defined curvature that enables the characterization of proteins with curvature sensing ability in...
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Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies10:22

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

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Procedures for complete reconstitution of a prototype voltage-gated potassium channel into lipid membranes are described. The reconstituted channels are suitable for biochemical assays, electrical recordings, ligand screening and electron crystallographic studies. These methods may have general applications to the structural and functional studies of other membrane...
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High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method07:26

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

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Bicelles are lipid/amphiphile mixtures that maintain membrane proteins (MPs) within a lipid bilayer but have unique phase behavior that facilitates high-throughput screening by crystallization robots. This technique has successfully produced a number of high-resolution structures from both prokaryotic and eukaryotic sources. This video describes protocols for generating the lipidic bicelle mixture, incorporating MPs into the bicelle mixture, setting up crystallizations trials (manually as well...
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Crystallization of Membrane Proteins in Lipidic Mesophases11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

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The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting...
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

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Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic...
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Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

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The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
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関連する実験動画

Updated: Jan 19, 2026

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
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High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

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膜タンパク質機能の形成における脂質の新たな役割

Rob Phillips1, Tristan Ursell, Paul Wiggins

  • 1Department of Applied Physics, California Institute of Technology, Pasadena, California 91125, USA. phillips@pboc.caltech.edu

Nature
|May 22, 2009
PubMed
まとめ

脂質環境は,膜タンパク質の構造と機能に直接影響を与えます. タンパク質によって引き起こされる膜の形状の変化は,エネルギーコストが定量化され,モデル予測が実験で確認されることが鍵です.

科学分野:

  • バイオフィジックス 生物物理学
  • メンブラン生物学 メンブラン生物学
  • タンパク質科学 タンパク質科学

背景:

  • 膜タンパク質は,周囲の脂質二層とダイナミックに相互作用する.
  • 脂質とタンパク質の相互作用は,タンパク質の構造と生物学的活動に影響します.
  • 特定の化学相互作用と物理的な膜変形がこれらの効果に貢献します.

研究 の 目的:

  • 膜タンパク質の機能における脂質環境の役割を調査する.
  • タンパク質によって引き起こされる膜の形状の変化に関連するエネルギーコストを定量化するために.
  • 実験データを用いて理論モデルを検証する.

主な方法:

  • 膜タンパク質とその脂質パートナーのモデルシステムを活用した.
  • タンパク質によって引き起こされる膜の乱れによる自由エネルギーコストを推定した.
  • 再構成されたシステムにおけるチャネルゲートダイナミクスの測定.

主要な成果:

  • 脂質環境と膜タンパク質の行動の間の直接的なリンクが確認されました.
  • タンパク質によって引き起こされる膜の形状の乱れが,機能に著しく影響することを示した.

さらに関連する動画

Crystallization of Membrane Proteins in Lipidic Mesophases
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

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関連する実験動画

Last Updated: Jan 19, 2026

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
07:26

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

Published on: January 9, 2012

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Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

31.8K
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

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  • 実験的な測定は,単純な生体物理モデルから得られた予測と一致した.

    • 結論:

    • 脂質二重層の物理的性質,特に膜の形状は,膜タンパク質の機能を決定する重要な決定因子です.
    • 量的な自由エネルギー計算は,脂質とタンパク質の相互作用に関する貴重な洞察を提供します.
    • モデルシステムは,複雑な膜タンパク質-脂質ダイナミクスを研究するのに有効です.