Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

A newly identified homologous SxtPR efflux system contributes to sulfamethoxazole/trimethoprim susceptibility and pathogenesis in <i>Klebsiella pneumoniae</i>.

Antimicrobial agents and chemotherapy·2026
Same author

Bacterial extracellular vesicles promote membrane repair and tolerance to polymyxin B.

Science advances·2026
Same author

A phage repressor involved in the DNA-damage response of <i>Francisella</i>.

PNAS nexus·2026
Same author

Large extrachromosomal replicons are widespread across bacterial lineages and show coordinated replication termination and spatial coupling with the chromosome.

Nature communications·2026
Same author

A novel GntR-ABC efflux system mediates oxidative stress response, drug resistance, motility and virulence in <i>Acinetobacter baumannii</i> ATCC 17978.

Frontiers in microbiology·2026
Same author

Role of the AmvAR efflux system on the pathogenesis of Acinetobacter baumannii.

Scientific reports·2026

関連する実験動画

Updated: Jun 23, 2026

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
09:37

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

SOS応答はインテグロン再結合を制御する.

Emilie Guerin1, Guillaume Cambray, Neus Sanchez-Alberola

  • 1Université de Limoges, Faculté de Médecine, EA3175, INSERM, Equipe Avenir, 87000 Limoges, France.

Science (New York, N.Y.)
|May 23, 2009
PubMed
まとめ

抗生物質耐性菌の拡散の鍵となるインテグロンは,細菌のSOS反応と関連しています. この結合は,細菌がストレス下での遺伝子カセット交換を制御することで適応するのを助け,抗生物質治療戦略に影響を与えます.

さらに関連する動画

Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

関連する実験動画

Last Updated: Jun 23, 2026

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
09:37

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

科学分野:

  • 微生物学 微生物学とは
  • 遺伝学 遺伝学とは
  • 分子生物学は分子生物学である.

背景:

  • インテグロンは,環境バクテリアに多く見られる遺伝子プラットフォームです.
  • 主に,抗生物質耐性遺伝子の拡散を促すことで,グラム陰性病原体の中で認識されています.

研究 の 目的:

  • インテグロンシステムと細菌のSOS応答の関係を調査する.
  • この相互作用がインテグロンインテグラーゼとカセット再結合の調節にどのように影響するかを理解する.

主な方法:

  • 遺伝子発現データの分析.
  • LexA.によるインテグロン・インテグラーゼの規制管理の調査
  • 細胞ストレスがインテグロン媒介による再結合に与える影響を評価する.

主要な成果:

  • SOS対応とインテグロン活動との間には直接的なリンクが確立されました.
  • LexAは,ほとんどのインテグロン・インテグラーゼの発現を制御する重要な調節因子として特定されました.
  • この規制コップリングは,ストレス条件下でのカセットキャプチャを強化し,安定した環境での再配置を最小限に抑えます.

結論:

  • インテグロンは,環境のシグナルに反応する統合された適応システムとして機能します.
  • インテグロンとSOS応答の相互作用は,抗生物質耐性を理解し,それと闘う上で重要な意味を持つ.
  • この発見は,将来の抗生物質治療政策に役立つかもしれない.