ATP-シトラートリアゼは,細胞代謝をヒストンのアセチル化と結びつける
Kathryn E Wellen1, Georgia Hatzivassiliou, Uma M Sachdeva
1Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104, USA.
Science (New York, N.Y.)
|May 23, 2009
まとめ
哺乳類ヒストンのアセチル化は,栄養素代謝と遺伝子発現を結びつけるアデノシン三酸塩 (ATP) - シトラートリアゼ (ACL) に依存しています. ACLは,グルコース由来シトラートをアセチル-CoAに変換し,ヒストンの改変に不可欠です.
科学分野:
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
背景:
- 単細胞エウカリオットはアセチル-CoA合成酵素を介してヒストンのアセチル化のためにアセチールを使用します.
- メタゾアンは主にグルコースを使用し,細胞外アセテートを制限しており,ヒストンアセチル化のための代替経路を必要とします.
研究 の 目的:
- 哺乳類の細胞におけるヒストンアセチル化に起因する主要な酵素を調査する.
- 栄養素の代謝をヒストンのアセチル化と遺伝子発現に結びつけるためのアデノシン・トリホスファート (ATP) - シトラートリアゼ (ACL) の役割を決定する.
主な方法:
- 哺乳類の細胞培養モデルを使用した.
- 異なるグルコース条件と成長因子刺激下でヒストンのアセチル化レベルを評価した.
- ATP-シトラートリアゼ (ACL) の酵素活性と,アセチル-CoA生成におけるその役割について調査した.
主要な成果:
- 哺乳類の細胞におけるヒストンのアセチル化は,ATP-シトラートリアゼ (ACL) に依存しています.
- ACLは,成長因子の刺激と分化中にヒストンのアセチル化が増加するために不可欠です.
- グルコースの可用性は,ACLに依存するメカニズムを通じてヒストンのアセチル化に影響します.
結論:
- ACLの活動は,成長因子誘発の栄養素代謝をヒストンアセチル化とリンクするために必要です.
- ACLは,細胞代謝を哺乳類の表遺伝子調節と遺伝子発現と結びつける重要な酵素として機能する.
関連する概念動画
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