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関連する概念動画

Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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関連する実験動画

Updated: Jun 22, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

数える合成遺伝子ネットワークです.

Ari E Friedland1, Timothy K Lu, Xiao Wang

  • 1Howard Hughes Medical Institute, Department of Biomedical Engineering, Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA.

Science (New York, N.Y.)
|May 30, 2009
PubMed
まとめ

科学者たちは,細胞カウンターとして機能するE. coliの合成遺伝子ネットワークを設計し,生物学的システムのプログラミングを可能にしました. これらの遺伝回路は最大3つの誘導イベントを数えることができ,先進的なバイオテクノロジーの応用への道を開く.

科学分野:

  • 合成生物学 合成生物学とは
  • 遺伝子工学 遺伝子工学とは
  • システム生物学 システム生物学

背景:

  • 合成遺伝子ネットワークは,数えるようなデジタルコンピューティングの原理を模倣して,細胞プログラミングを行うことができます.
  • セルラーカウンターは,合成プログラミングとバイオテクノロジーの進歩に不可欠です.
  • Escherichia coliは,新しい遺伝回路を開発するためのモデル生物として機能します.

研究 の 目的:

  • Escherichia coli. の2つの異なる合成遺伝カウンターを設計し,特徴づけること.
  • 誘導イベントを追跡するこれらのカウンターの能力を実証するために.
  • スケーラブルなカウントアプリケーションのためのモジュラー遺伝子デバイスを確立する.

主な方法:

  • 数えるためのリボレギュレーションされた転写カスケードの構築.
  • 数えるためのメモリユニットの再結合酵素ベースのカスケードの開発.
  • Escherichia coli. の合成カウンターのテストと検証

主要な成果:

  • 3つの誘導イベントまで数えることができる2つの補完的な合成遺伝子カウンターを成功裏に作成しました.
  • これらのモジュラーデバイスが,ユーザーによって定義されたさまざまな入力数を数えることができることを実証しました.

さらに関連する動画

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

関連する実験動画

Last Updated: Jun 22, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

  • リボレギュレーションと再結合酵素ベースのカウントシステムの機能性を検証した.

    • 結論:

    • 合成遺伝子ネットワークは,機能的な細胞カウンターとして設計することができます.
    • 開発されたカウンターはモジュール式であり,より大きな数のカウントのために拡張できます.
    • これらの遺伝子カウントデバイスは,複雑な合成生物学アプリケーションの可能性を秘めています.