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Updated: Jun 22, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

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転写の構造的基礎: 3.4 アングストームの解像度で逆行RNAポリメラーゼII

Dong Wang1, David A Bushnell, Xuhui Huang

  • 1Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Science (New York, N.Y.)
|May 30, 2009
PubMed
まとめ

研究者らは,RNAポリメラーゼIIの逆行状態の結晶構造を決定し,校正とRNA分裂に不可欠な特定の結合部位を明らかにした. この発見は,転写の忠誠性についての理解を深める.

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Transcription Initiation01:47

Transcription Initiation

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All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Bacterial Transcription01:53

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RNA Polymerase II Accessory Proteins02:36

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...