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関連する概念動画

Redox Reactions01:27

Redox Reactions

Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
Redox Reactions01:24

Redox Reactions

Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
Redox Equilibria: Overview01:23

Redox Equilibria: Overview

A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...

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関連する実験動画

Updated: Jun 22, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

リボヌクレオチド還元酵素のレドックス関連構造変化

A R Offenbacher1, I R Vassiliev, M R Seyedsayamdost

  • 1Department of Chemistry and Biochemistry and the Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.

Journal of the American Chemical Society
|June 4, 2009
PubMed
まとめ
この要約は機械生成です。

リボヌクレオチド還元酵素 (RNR) は,チロシル基 (Y122*) を用いて,触媒作用を開始する. この研究は,近隣のアミド結合のリドックス関連構造変化を明らかにし,それは,根幹の静電的なシフトによって引き起こされる.

さらに関連する動画

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

関連する実験動画

Last Updated: Jun 22, 2026

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • スペクトロスコーピーは,スペクトロスコーピーを用います.

背景:

  • リボヌクレオチド還元酵素 (RNR) は,DNA合成に不可欠であり,デオキシリボヌクレオチド生成を触媒化する.
  • クラスIのRNRは,E. coli beta2サブユニットと同様に,鉄中心と,触媒作用のための重要なチロシルフリーラジカル (Y122*) を特徴としています.
  • Y122*のレドックス依存型構造の変化は,陽子結合電子伝送を調節する可能性がある.

研究 の 目的:

  • E. coli beta2.2のY122*ラジカルに関連したレドックス関連構造変化を調査する.
  • 触媒反応を開始するチロシルラジカルの役割を理解する.
  • 陽子結合電子伝送調節のメカニズムを探求する.

主な方法:

  • フーリエ変換赤外線 (FT-IR) スペクトロスコピーは,反応誘発のスペクトル変化を検出するために使用されました.
  • (2) H (((4) ティロシンと (15) N ティロシンによる同位体ラベル付けは,スペクトルの割り当てを助けました.
  • 差異スペクトルは,Y122*をヒドロキシウレアで還元する過程で得られた.

主要な成果:

  • FT-IR分析により,Y122 (1514 cm(-1)) とY122* (1498 cm(-1)) と関連した特定の振動帯が特定されました.
  • 反応誘発スペクトルは,アミドI帯 (1661と1652cm(-1) の変化を示し,構造的変化を示した.
  • これらのアミド帯のシフトは,モデルペンタペプチドで観察されたシフトを反映し,配列媒介効果を示唆しました.

結論:

  • Y122*の減少は,近くのアミド結合の構造的混乱と結びついている.
  • これらの構造の変化は,チロシル基を囲むアミノ酸配列の影響を受けます.
  • 提案されたメカニズムは,チロシルラジカルのアロマティックリング内のレドックス結合電気静的変化が,アミド結合の混乱を誘発することを含む.