DNAポリメラーゼVの活性形態は,UmuD'(2)C-RecA-ATPというものです
Qingfei Jiang1, Kiyonobu Karata, Roger Woodgate
1Department of Biological Sciences, University of Southern California, University Park, Los Angeles, California 90089-2910, USA.
Nature
|July 17, 2009
まとめ
RecA*は,RECA-ATPをDNAポリメラーゼV (pol V) に転送し,活性変異体を作り,トランスレションDNA合成を可能にします. このミュータソームは,ReCA*から独立して機能し,その活動はReCA-ATP交換によって規制されます.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 遺伝学 遺伝学とは
背景:
- Escherichia coliにおけるSOS変異は,DNAポリメラーゼV (pol V) とRecA核タンパク質フィラメント (RecA*) を含む.
- アクティブポルVの正確な分子組成と,ミュータゲネシスにおけるReCA*の正確な機能は不明のままである.
研究 の 目的:
- ポルVの活性変異性形態の分子組成を解明する.
- SOS変異遺伝のためのpol Vを活性化するReCA*の特定の役割を定義する.
主な方法:
- ポリV Mut複合体の生化学的再構成.
- RecA-ATP転送のステキオメトリック分析.
- ポリV Mutの活性と非活性化に関する運動学的研究.
主要な成果:
- RecA*は単一のReCA-ATP分子をフリーポリV (UmuD'(2) Cに移し,UmuD'(2) C-ReCA-ATP組成の活性ミュータソーム (pol V Mut) を形成する.
- Pol V Mutは,ReCA*とは独立してトランスレション合成 (TLS) を触媒化する.
- Pol V MutはDNA解離時に迅速に無効化し,DNA合成が欠けるとよりゆっくりと無効化し,RECA-ATPを保持します.
結論:
- SOS突然変異におけるReCA*の主な役割は,ReCA-ATPをポリVにステキオメトリック的に転送することです.
- この移転により,トランスレション合成に不可欠な活性変異体複合体が生成されます.
- Pol V Mutの活動は,RECA-ATPの交換によって調節され,突然変異を制御するための新しいメカニズムを強調しています.
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