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Updated: Jun 21, 2026

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
07:39

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Published on: April 13, 2022

異なる特異性のソルターゼを使用した単一のポリペプチドのサイト固有のNおよびC端末ラベル付け

John M Antos1, Guo-Liang Chew, Carla P Guimaraes

  • 1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Journal of the American Chemical Society
|July 21, 2009
PubMed
まとめ

この研究では,2つのソルターゼ酵素を使用して,両方の端で精密なタンパク質ラベル付けを行っています. Staphylococcus aureus (SrtA ((staph)) とStreptococcus pyogenes (SrtA ((strep)) のソルターゼAは,単一のポリペプチドのサイト固有の改変を可能にします.

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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

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