微生物燃料電池におけるリドックス酵素の表面表示

Simon Fishilevich¹, Liron Amir, Yearit Fridman

¹Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel.

Journal of the American Chemical Society

|August 12, 2009

まとめ

この研究は,酵素を継続的に生産するために工学酵母を使用した新しいバイオ燃料電池を提示し,長期の動作のために安定性と出力力を高めます.

科学分野:

バイオテクノロジーバイオテクノロジー
バイオ電気化学バイオ電気化学
酵素工学とは

背景:

酵素の不安定さは,従来のバイオ燃料電池の運用寿命を制限する.
継続的な生物触媒の生産は,バイオ燃料電池の持続的な性能に不可欠です.
微生物燃料電池は,再生可能エネルギー発電の有望な道を提供します.

研究の目的:

酵母をエンジニアリングして活性酵素を表示する安定で効率的なバイオ燃料電池を開発する.
バイオ燃料電池の性能を改善するために,バイオカタリストの生産と電気化学的活動を強化します.
設計されたバイオ燃料電池の性能を既存の技術と比較する.

主な方法:

酵母表面ディスプレイシステムを使用して,Saccharomyces cerevisiaeのAspergillus nigerグルコース酸化酵素を表現しました.
生化学的および電気化学的測定によって酵素活性が実証されています.
ハイブリッドバイオ燃料電池を製造し,アノドに酵母,カソドにラカゼを組み立てました.

主要な成果:

改造酵母は,改造されていない酵母と精製された酵素と比較して,著しく高いグルコース酸化酵素活性を示した.
構築されたバイオ燃料電池は,優れた出力と電流密度を示した.
バイオ燃料電池は,精製された酵素を使用した細胞よりも著しく長い稼働時間を実証しました.

結論:

活性酵素を表示する人工酵母は,バイオ燃料細胞開発のための安定的かつ効率的なプラットフォームを提供します.
このハイブリッドアプローチは,酵素の不安定性の問題を克服し,バイオ燃料電池の長時間稼働を可能にします.
開発されたバイオ燃料電池は,再生可能エネルギー技術の重要な進歩を表しています.

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