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関連する概念動画

Overview of Myosin Structure and Function01:15

Overview of Myosin Structure and Function

Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with their role in muscle contraction, these proteins also play a role in the intracellular transport of molecules and vesicles. There are twenty-four classes of myosins based on their domain sequence and organization. Of the twenty-four, six classes (Myosin I, Myosin II, Myosin V, Myosin VI, Myosin VII, and Myosin X)  have been well characterized.
Microtubule Associated Motor Proteins01:32

Microtubule Associated Motor Proteins

Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular cargos...
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Actin and Myosin in Muscle Contraction01:16

Actin and Myosin in Muscle Contraction

Actin and myosin are contractile proteins that form the sarcomere found in skeletal muscle tissues for regulating muscle contraction. Actin, a globular contractile protein, interacts with myosin for muscle contraction. The skeletal tissue appears striped or striated under a microscope due to the repeated arrangement of contractile proteins actin and myosin along the length of myofibrils. Dark A bands and light I bands repeat along myofibrils, and the alignment of myofibrils in the cell causes...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

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関連する実験動画

Updated: Jun 21, 2026

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
08:57

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Published on: February 4, 2021

ミオシンVIは,貨物媒介による二分化を受けます.

Cong Yu1, Wei Feng, Zhiyi Wei

  • 1Department of Biochemistry, Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.

Cell
|August 12, 2009
PubMed
まとめ
この要約は機械生成です。

ミオシンVIモータータンパク質は,Dab2のような荷物を結合すると,モノマーからダイマーに変換されます. この貨物結合に起因する二分化が,アクチン繊維に沿ったマイナスエンド方向のユニークな動きを調節する.

さらに関連する動画

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
08:06

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Published on: February 14, 2016

関連する実験動画

Last Updated: Jun 21, 2026

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
08:57

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Published on: February 4, 2021

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
08:06

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Published on: February 14, 2016

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物物理学 細胞生物物理学
  • 構造生物学 構造生物学とは

背景:

  • ミオシンVIは,様々な細胞機能に不可欠なアクチン繊維のマイナス端に向かって動くユニークな分子モーターです.
  • ミオシンVIの過程的動きは二分化に依存しているが,モノマー-二分子の変換のメカニズムは不明である.

研究 の 目的:

  • ミオシンVIにおけるモノマー・ダイマー変換を調節する分子メカニズムを解明する.
  • ミオシンVIの機能的調節における貨物結合の役割を調査する.

主な方法:

  • cargo-free Myosin VI cargo-binding domain (CBD) の高解像度 NMR 構造を決定しました.
  • ミオシンVI CBDとDab2タンパク質の断片の相互作用を特徴づけた.
  • ミオシンVICBD-Dab2複合体のX線構造を解明しました.

主要な成果:

  • 貨物なしのMyosin VI CBDは,溶液中の安定したモノマーとして存在します.
  • ミオシンVICBDは,アダプタタンパク質Dab2の断片に高親和度で結合する.
  • X線結晶学により,貨物結合がミオシンVICBDの二分化を引き起こすことが明らかになった.

結論:

  • 荷重結合誘発型二酸化は,ミオシンVIの活性とプロセシビティを調節する重要なメカニズムです.
  • この二分化パラダイムは,ミオシンVIIやミオシンXなどの他のミオシンにも適用される可能性があります.