ムシンのグリコプロテインによって促進される有機反応
Natalie Shraga1, Bogdan Belgorodsky, Michael Gozin
1School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel.
Journal of the American Chemical Society
|August 14, 2009
まとめ
ムキン,天然のタンパク質は,エステル水解やダイエルス・アルダー反応のような化学反応を意外と加速します. この発見は,非酵素触媒とバイオマテリアルの応用に新たな可能性をもたらします.
科学分野:
- バイオケミストリー バイオケミストリー
- 有機化学 オーガニック・ケミストリー
- マテリアルサイエンス 材料科学
背景:
- ムキンは,水害性の有機物質を結合することが知られている広範にグリコシル化されたタンパク質です.
- 以前の研究は,化学反応を触媒化するムシンの能力を確立していなかった.
研究 の 目的:
- 有機反応を促進するムシンの触媒的能力を調査し,実証する.
- 水嫌性基質のための天然の非酵素触媒としてのムシンの可能性を調査する.
主な方法:
- 脂肪酸p-ニトロフェノールエステル水解の加速を,牛のサブマキシラームシンと豚の胃ムシンを用いて評価した.
- 粘膜の存在下でN-プロピルマレイミドとアントラセンの間のダイエルス・アルダー反応の速度増幅を測定した.
主要な成果:
- ムシンは,脂肪酸p-ニトロフェノールエステルの水解を最大337倍まで加速させた.
- ムキンは,基準条件と比較して,ディエルス・アルダー反応の速度を最大200倍まで増加させた.
結論:
- この研究は,ムシンが有機反応を加速し,天然の非酵素触媒として作用する最初の証拠を提供します.
- 水溶液中の性物質との反応を促進するムシンの能力は,薬物投与およびバイオ材料における新しい応用を提供します.
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