新しいオクタマー結合トランスクリプション因子は,マウスの胚細胞で差異的に発現しています

K Okamoto¹, H Okazawa, A Okuda

¹Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.

Cell

|February 9, 1990

まとめ

研究者らは,P19幹細胞で新しい転写因子,オクトマー結合因子 (Oct-3) を発見した. その発現は微分化時に減少し,マウスの胚発達における役割を示唆している.

科学分野:

発達生物学発達生物学とは
分子生物学は分子生物学である.
遺伝学遺伝学とは

背景:

P19胚性がん細胞は,マウスの早期発達のモデルとして機能する.
オクタマー結合因子は,遺伝子調節において重要な役割を果たします.

研究の目的:

P19幹細胞における新しいオクターマー結合因子を特定し,特徴づけること.
レチノ酸誘発分化におけるこの因子の役割を調査する.

主な方法:

オクトマー結合因子 (Oct-3) cDNAの識別とクローン.
Oct-3タンパク質の構造とDNA結合特性に関する分析.
微分化中のOct-3 mRNA発現の定量分析.

主要な成果:

P19幹細胞に新しいオクトマー結合因子Oct-3が同定されました.
Oct-3は,オクターマーモチーフを含む特定のDNAモチーフを認識します.
Oct-3の発現は,レチノ酸誘発の微分化によってダウンレギュレーションされます.
Oct-3 mRNAは成人マウスの臓器では検出できない.

結論:

Oct-3は,P19細胞の幹細胞状態を維持するために不可欠な発達的に調節される転写因子です.
その独特のPOUドメインとN端末のアクティベーションドメインは,ユニークな規制機能を示唆しています.
Oct-3はマウスの胚形成と幹細胞の分化に重要な役割を果たしている可能性が高い.

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