関連する実験動画
Updated: May 11, 2026
09:41
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
Published on: June 18, 2014
サイクリン (Cyclin) は,クセノプス (Xenopus) の成熟促進因子の成分である
J Gautier1, J Minshull, M Lohka
1Department of Pharmacology, School of Medicine, University of Colorado, Denver 80262.
Cell
|February 9, 1990
まとめ
クセノプスの卵の成熟促進因子 (MPF) には,サイクリンB1とB2の両方が含まれています. これらのサイクリンとp34cdc2は,卵細胞の成熟と卵子活性化中の細胞サイクル進行を駆動する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学とは
背景:
- 成熟促進因子 (MPF) は,卵細胞の細胞サイクル進行に不可欠です.
- MPFの正確な成分は,調査の対象となっている.
研究 の 目的:
- Xenopusの卵から高度に精製されたMPFの成分を特定するために.
- MPF活動におけるB型サイクリンの役割を明らかにする.
主な方法:
- 抗B型サイクリン抗体を用いたウェスタン・ブラッティングと免疫降水.
- 免疫プレシピテートにおけるヒストンH1キナーゼ活性測定.
- 熟成中の卵細胞と活性化卵のタンパク質キナーゼ活性をモニタリングする.
主要な成果:
- 高精製されたMPFには,サイクリンB1とサイクリンB2の両方が含まれています.
- 免疫プレシピテートはヒストンH1キナーゼ活性を示し,サイクリンB1とB2が基質として機能した.
- サイクリン誘導タンパク質キナーゼ活性が,卵細胞の成熟と卵子活性化中のp34cdc2キナーゼ活性に反映された.
結論:
- B型サイクリン (B1およびB2) は,Xenopus.のMPFの不可欠な成分である.
- MPFの活動は,p34cdc2とB型サイクリンとの相互作用によって調節されます.
関連する概念動画
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:02Meiosis II
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...