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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

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関連する実験動画

Updated: Jun 20, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

トランポゾンターゲティングを絞り込む.

Mick Chandler1

  • 1Laboratoire de Microbiologie et Génétique Moléculaire UMR 5100, CNRS, 31062 Toulouse CEDEX, France. mike@ibcg.biotoul.fr

Cell
|August 26, 2009
PubMed
まとめ
この要約は機械生成です。

Escherichia coli のスライディングベータクランプは,TnsE タンパク質と相互作用して,Tn7 トランポソンをDNA 複製フォークに誘導します. この調整は,ゲノム複製の過程でトランスポーゼーションを助けます.

さらに関連する動画

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

関連する実験動画

Last Updated: Jun 20, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

科学分野:

  • 分子生物学は分子生物学である.
  • バクテリアの遺伝学
  • DNAの複製と修復を図っています.

背景:

  • スライディングベータクランプは,Escherichia coliのDNA複製機構の重要な構成要素です.
  • ゲノム複製における役割を超えて,ベータクランプは複数の細胞機能を調整します.

研究 の 目的:

  • ベータクランプと転置タンパク質TnsEの相互作用を調査する.
  • この相互作用がTn7トランポゾンを特定のDNAの位置にどのようにターゲットにするかを決定する.

主な方法:

  • この研究では,タンパク質とタンパク質の相互作用を証明するための生化学的測定法が含まれていた可能性が高い.
  • 複製フォークのトランポジションイベントを視覚化または追跡するためのテクニックがおそらく使用されました.

主要な成果:

  • パークス・エット・アル. (2009) は,βクランプが転置タンパク質TnsE.と相互作用することを発見しました.
  • この相互作用は,特異的にTn7トランポゾンを標的にして,複製フォークで不連続に複製するDNAを誘導します.

結論:

  • ベータクランプは,Tn7の転移を活性複製部位に誘導する上で重要な役割を果たします.
  • これは,DNA複製と転置機構の間の新しい調整を強調しています.