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The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

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関連する実験動画

Updated: Jun 20, 2026

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

複製DNAへの変換は,プロセス性因子との相互作用によって起こる.

Adam R Parks1, Zaoping Li, Qiaojuan Shi

  • 1Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.

Cell
|August 26, 2009
PubMed
まとめ
この要約は機械生成です。

細菌のトランスポソンTn7は,複製DNAを標的としてタンパク質TnsEを使用しています. TnsEはDNA複製機構と相互作用し,活性複製部位への移植を誘導する.

さらに関連する動画

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

関連する実験動画

Last Updated: Jun 20, 2026

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • 微生物学 微生物学とは

背景:

  • 細菌のトランポゾンTn7は,活性な複製DNAに挿入することを好む.
  • このトランポジションは,トランポゾンでエンコードされたタンパク質TnsEによって媒介されます.

研究 の 目的:

  • TnsEと宿主DNA複製機構の相互作用を調査する.
  • Tn7が積極的に複製するDNAを標的とするメカニズムを解明する.

主な方法:

  • タンパク質とタンパク質の相互作用を研究するために,in vivoおよびin vitroの生化学測定法.
  • 精製されたタンパク質とDNAを用いたTnsABC+Eトランポジション反応の復元.
  • DNA複製に関連した転置部位選択の分析.

主要な成果:

  • TnsEは,物理的に,機能的にDNA複製プロセス性因子と相互作用します.
  • in vitroトランスポーゼーションシステム (TnsABC+E) が成功裏に再構成されました.
  • プロセシビティファクターは,TnsE媒介のトランポジションイベントをリオーダーし,in vivoレプリケーションバイアスを反映することが示されました.

結論:

  • TnsEと複製機械の相互作用は,Tn7のターゲットサイト選択の鍵です.
  • このメカニズムは,Tn7が積極的に複製するDNAに優先的にトランスポーズすることを可能にします.
  • 同様のメカニズムは,他の移動性遺伝子要素によって採用されることがあります.