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関連する概念動画

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
The DNA Helix01:16

The DNA Helix

Overview
The DNA Helix01:07

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

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関連する実験動画

Updated: Jun 20, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
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Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

2次元のDNA配列: 3層のログパイル

Jonathan Malo1, James C Mitchell, Andrew J Turberfield

  • 1Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK.

Journal of the American Chemical Society
|September 3, 2009
PubMed
まとめ
この要約は機械生成です。

研究者らは,合成結晶の構造的な秩序を強化するために,新しい3層のログパイル (3LL) DNA配列を開発しました. このDNAの自己組み立て方法は,広範囲にわたる,秩序付けられたシートを生成し,先進的な材料への道を開く.

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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

関連する実験動画

Last Updated: Jun 20, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

科学分野:

  • 合成生物学 合成生物学とは
  • ナノテクノロジー ナノテクノロジー
  • バイオフィジックス 生物物理学

背景:

  • DNAの自己組み立ては,オーダーされたナノ構造を作り出すための経路を提供します.
  • 既存のDNA配列は,構造的整合性と順序に制限がある可能性があります.
  • 周期的なテンプレートは,合成タンパク質結晶化などのアプリケーションにおいて極めて重要です.

研究 の 目的:

  • 新しい3層のログパイル (3LL) DNA配列の設計と自己組み立てを記述する.
  • 構造的な順序と連続ヘリクスを最大化したDNA配列を作成します.
  • 組み立てプロセスと,その結果生じるナノ構造を特徴付ける.

主な方法:

  • 4つの合成オリゴヌクレオチドとホリデー交差点モチーフを使用した二次元DNA配列の設計.
  • アセンブリの運動学とヒステレシスを研究するためのUV吸収度測定.
  • 構造的視覚化のためのネガティブステイン伝送電子顕微鏡 (TEM).
  • 繰り返し相関マッピングとTEMマイクログラフの平均化.

主要な成果:

  • 3層のログパイル (3LL) DNA配列の自己組み立てが成功しました.
  • 紫外線吸収による3LL組立中の速度依存ヒステリシスの観察.
  • TEM.で観測された3LL構造の広大なシート (マイクロメートルスケール) の形成.
  • 平均化されたTEM投影画像は,結晶構造のコンピュータ生成モデルを検証した.

結論:

  • 3層のログパイル (3LL) は,高い構造秩序を持つ安定した二次元DNA配列である.
  • 連続ヘリックスデザインは,オーダーされたテンプレート化の可能性を高めます.
  • このDNA配列は,合成材料と結晶学におけるアプリケーションの重要な進歩を表しています.