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Updated: Jun 20, 2026

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters
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Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters

Published on: May 29, 2026

3'-端末の高精度ベースペアリング

Amritraj Patra1, Clemens Richert

  • 1Institute for Organic Chemistry, University of Karlsruhe (TH), 76131 Karlsruhe, Germany.

Journal of the American Chemical Society
|September 3, 2009
PubMed
まとめ

新しい2'-アントラキノイラミド-2'-デオキシウリジン (Uaq) 分子キャップは,DNAとRNAの結合親和性とプローブ3'-末端での選択性を高めます. このアシレートされたアミノヌクレオシドは,高精度ハイブリダイゼーションアプリケーションに優れた性能を提供します.

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Proofreading01:43

Proofreading

Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...