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Updated: Jun 20, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
12:08

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

Streptomyces thioluteusからのアミン酸素酶,AurFの長寿,基板-水酸化ペロキシジロン ((III/III) の中間物質である

Victoria Korneeva Korboukh1, Ning Li, Eric W Barr

  • 1Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.

Journal of the American Chemical Society
|September 8, 2009
PubMed
まとめ

アミン酸化酵素AurF酵素は,p-アミノベンゾ酸 (pABA) を酸化するために安定したペロキソ-Fe(2)(III/III) 中間物質を使用します. この中間物質は迅速にpABAを酸化し,p-ニトロベンゾ酸 (pNBA) に完全な変換におけるその役割を示唆しています.

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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory08:02

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Published on: August 23, 2018

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