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関連する概念動画

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

芽生える酵母菌のRNAi

Ines A Drinnenberg1,2, David E Weinberg1,2,3, Kathleen T Xie1,2,3

  • 1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|September 12, 2009
PubMed
まとめ
この要約は機械生成です。

RNA干渉 (RNAi) は,Saccharomyces castelliiのような芽生えた酵母に存在し,新しいDicerタンパク質を使用しています. Saccharomyces cerevisiaeでRNAiを再構成すると,レトロトランポゾンが静止され,新しい研究ツールが提供されます.

さらに関連する動画

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

関連する実験動画

Last Updated: Jun 20, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • イースト生物学 イースト生物学

背景:

  • RNA干渉 (RNAi) は,真核生物における保存された遺伝子静止機構である.
  • RNAi経路は,モデル生物であるSaccharomyces cerevisiaeに顕著に欠けていた.
  • 以前の研究では,ある種の芽生えた酵母種においてRNAiが失われていることが示されていた.

研究 の 目的:

  • Saccharomyces cerevisiae以外の芽生えた酵母種におけるRNAiの存在とメカニズムを調査する.
  • これらの種におけるRNAiに関与する特定のタンパク質を特定する.
  • 遺伝子サイレンシングの研究のために,Saccharomyces cerevisiaeのRNAiを再構成する可能性を調査する.

主な方法:

  • Saccharomyces castelliiとCandida albicansにおけるRNAi経路の構成要素の比較ゲノム学と分子解析について.
  • 非正規のDicerタンパク質の識別と特徴付け.
  • RNAiの活性と標的遺伝子の静止を評価するための機能分析.
  • Saccharomyces cerevisiaeの遺伝子操作により,S. castelliiからRNAi成分を導入し,テストする.

主要な成果:

  • RNAiはSaccharomyces castelliiとCandida albicansで検出され,非正規のDicerタンパク質を使用しました.
  • これらの種で生成される小型の干渉RNAは,主に転移可能な元素とY'亜テロメア繰り返しを標的とする.
  • S. castelliiのRNAi欠乏変異体はY'メッセンジャーRNAレベルが上昇した.
  • S. castelliiの導入により,Dicer and ArgonauteはSaccharomyces cerevisiaeにRNAiを回復させ,内生レトロトランポゾンを静止させました.

結論:

  • RNAiで機能するDicerタンパク質の新種のクラスは,芽生えた酵母で特定されました.
  • この研究では,芽生えた酵母における遺伝子静止を調査するためのRNAiツールを再導入しています.
  • この研究は,芽生えた酵母系をRNAiメカニズムを研究するために適用することを可能にします.