エクソソームは,転写後のネガティブなフィードバックループで昼夜間の遺伝子発現を調節する
Jinhu Guo1, Ping Cheng, Haiyan Yuan
1Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Cell
|September 15, 2009
まとめ
FFC複合体は,exosome経由でfrqRNAの分解を制御することによって,昼夜リズムを調節する. この転写後のフィードバックループは,時計遺伝子発現の堅固なリズム性を保証します.
科学分野:
- 分子生物学は分子生物学である.
- シルカディアン生物学
- 遺伝子規制 遺伝子規制
背景:
- ユカリオットの昼間振動器は,負のフィードバックループに依存しています.
- これらの時計における転写後のRNA調節の役割はほとんど不明である.
- ニューロスポラでは,FFC複合体は frq 転写を抑制する.
研究 の 目的:
- ニューロスポラの昼夜時計における転写後の調節の役割を調査する.
- FFC複合体がRNAの安定性に影響するかどうかを判断する.
- frq RNAのリズム性を制御するメカニズムを解明する.
主な方法:
- FFC-RNAの相互作用を検出するためのRNA免疫プレシピテーション (RIP).
- RRP44.4を静音化することでエクソソーム機能測定を行う.
- 異なる条件下でfrqRNAとタンパク質のレベルを分析する.
- ChIPは,White Collar複合体の rrp44への結合を評価する試験を行っています.
主要な成果:
- FFCはfrq RNAと結合し,エクソソームと相互作用し,frq RNAの分解を促進する.
- frq RNAは,FRQレベルが低い場合,より高い安定性を持つ,堅固なリズム性を表しています.
- RRP44を静止すると,frqRNAレベルが上昇し,クロック機能が妨げられます.
- rrp44は時計制御遺伝子であり,ホワイトカラー複合体の直接標的であり,他の時計制御遺伝子を調節する.
結論:
- FFCとエクソソームは, frq RNAレベルを調節するポストトランスクリプションの負のフィードバックループを形成します.
- このメカニズムは,堅固な昼夜リズムとクロック出力を維持するために不可欠です.
- 転写後の制御は,真核生物の昼間振動器において重要な役割を果たします.
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