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インスリン依存型糖尿病のベータ細胞抗原としての熱ショックタンパク質65
D B Jones1, N R Hunter, G W Duff
1Department of Medicine, Western General Hospital, Edinburgh, UK.
Lancet (London, England)
|September 8, 1990
まとめ
Mycobacterium tuberculosisからの熱ショックタンパク質65 (HSP65) は,インスリン依存型糖尿病における主要なベータ細胞抗原である可能性があります. HSP65に対する自己免疫は,この自己免疫疾患に対する感受性を高める可能性があります.
科学分野:
- 免疫学 免疫学とは
- 内分泌学 エンドックリノロジー
- 微生物学 微生物学とは
背景:
- インスリン依存型糖尿病における主要なベータ細胞抗原は,約64kDのタンパク質である.
- インスリン腫細胞は,この64 kDのタンパク質を,高温とインタールイキン-1β,ガンマインターフェロン,腫瘍死滅因子などのサイトカインを刺激すると合成します.
研究 の 目的:
- 64 kDのベータ細胞抗原のアイデンティティを調査するために.
- インスリン依存型糖尿病の病原性における熱ショックタンパク質の潜在的な役割を調査する.
主な方法:
- ウェスタン・ブラット分析は,Mycobacterium tuberculosisとMycobacterium lepraeの64 kDのベータ細胞タンパク質と熱ショックタンパク質65 (HSP65) に対する抗体間のクロス反応性を評価するために使用されました.
- 抑制アッセイは,インスリン依存型糖尿病患者の血清を用いて実施した.
主要な成果:
- 64kDのベータ細胞タンパク質と,Mycobacterium tuberculosis HSP65に対する抗体との間にはクロスリアクティビティが観察されたが,Mycobacterium leprae HSP65.5に対する抗体は観察されなかった.
- インスリン依存型糖尿病患者の血清は,64 kDのベータ細胞抗原に対する抗体を含んでおり,Mycobacterium tuberculosis HSP65抗体のサイトカイン治療細胞への結合を抑制しました.
結論:
- 熱ショックタンパク質65 (HSP65) は,64 kDのβ細胞抗原であると示唆されています.
- HSP65のエピトープに対する自己活性性は,インスリン依存型糖尿病への感受性を引き起こす可能性があります.
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