乳がん発生に染色体17pの少なくとも2つの遺伝子が関与している証拠
C Coles1, A M Thompson, P A Elder
1Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Lancet (London, England)
|September 29, 1990
まとめ
研究者らは,乳がん患者で染色体17の異性交性の喪失をマッピングした. 17p13-3のp53遺伝子の近くの調節遺伝子は,ほとんどの乳がんに関与しています.
科学分野:
- 遺伝学 遺伝学とは
- 腫瘍学 腫瘍学
- 分子生物学は分子生物学である.
背景:
- 染色体17の異質性喪失 (LOH) は,様々なヒトがんで頻繁に観察されています.
- 染色体17p13.1に位置するp53遺伝子は,重要な腫瘍抑制遺伝子である.
- 以前の研究では,染色体17が乳がんの発症に関与していることが示されていた.
研究 の 目的:
- 乳がん患者の大規模なコホートにおける染色体17のLOHの領域を特定し,マッピングする.
- 染色体17の特定の位置におけるLOHと乳がんとの関係を調査する.
- 乳がんの病原性におけるp53へのテロメリック調節遺伝子の潜在的な役割を調査する.
主な方法:
- 乳がん患者のペア化した腫瘍と血液白血球サンプルのDNA分析.
- マイクロサテライト分析で,染色体17のヘテロジゴシティの喪失を検出する.
- p53 mRNA発現の定量分析. p53 mRNA発現の定量分析. p53 mRNA発現の定量分析. p53 mRNA発現の定量分析.
主要な成果:
- 17p染色体で確認されたLOHの高頻度.
- 情報提供性腫瘍の3分の1は,長い腕のTHH59の場所でのアレル喪失を示した.
- ショートアームで2つの異なるLOH領域が特定されました: 17p13-3と17p13-1 (p53を含む可能性が高い).
- 17p13-3領域は,17p13-1と比較して,LOHの頻度が著しく高かった.
- 2つの部位でのアレル喪失との相関は観察されなかった.
- 17p13-3のLOHは,p53 mRNA過剰発現と関連していました.
結論:
- 17p13-3のLOHは乳がんに多く見られ,p53 mRNAの過剰発現と関連しています.
- 17p13-3のp53へのテロメリック約20メガベースの調節遺伝子は,乳がんに関与しているようです.
- この調節遺伝子の病変は,ほとんどの乳がん症例において重要な役割を果たす可能性があります.
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