関連する実験動画
Updated: Aug 7, 2026
12:13
Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
Published on: August 23, 2014
3種類の下垂体細胞が欠けている矮体ロカス変異体は,POUドメインの遺伝子ピット-1の変異から生じる
S Li1, E B Crenshaw, E J Rawson
1Department of Chemistry, University of California, San Diego, La Jolla 92093.
Nature
|October 11, 1990
まとめ
ネズミの矮小部分の変異は,前垂体発達の発達を妨害する. POUドメインの転写因子Pit-1は,成長ホルモン,プロラクチン,甲状腺刺激ホルモン産生細胞の発達に不可欠である.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- エンドクリノロジー エンドクリノロジー
背景:
- マウス・ダワーフ・ロカス (dw) は前垂体発育不全と関連しています.
- これらの欠陥には,成長ホルモン,プロラクチン,甲状腺刺激ホルモンの発現の喪失が含まれます.
- これらの欠陥の細胞基盤は,ホルモンを産生する細胞タイプの低増殖を伴う.
研究 の 目的:
- マウス・ダワーフ・ロカス変異の遺伝的基礎を調査するために.
- 前部下垂体発育におけるPit-1遺伝子の役割を決定する.
- 転写因子機能と哺乳類の臓器生成を結びつけるために.
主な方法:
- マウス・ダワーフ・アレルの遺伝子解析.
- 下垂体ホルモンの遺伝子発現の研究.
- 前部下垂体細胞のタイプのフェノタイプ分析.
主要な成果:
- 矮小部分の突然変異は前垂体発達の発達を妨げます.
- 両方の特徴化された矮小アレルは,Pit-1遺伝子における障害を含んでいる.
- Pit-1は,成長ホルモン,プロラクチン,甲状腺刺激ホルモン産生細胞の特異化と発達に不可欠です.
結論:
- Pit-1は前垂体細胞の分化のための重要な転写因子です.
- Pit-1遺伝子は,矮星の位置に直接関与しています.
- この研究は,特定の転写因子を哺乳類の臓器形成における重要な出来事と関連付けています.
さらに関連する動画
関連する概念動画
01:33Pleiotropy
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
02:41Lethal Alleles
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:17The Pituitary Gland
The pituitary is a small endocrine organ in the sphenoid bone under the hypothalamus. Primarily, the pituitary in adults has two distinct anatomical and functional regions— the anterior and posterior lobes. During human fetal development, a third pituitary gland region called the pars intermedia atrophies and disappears. However, some of its cells migrate and exist adjacent to the anterior pituitary in adults.