Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Real Time RT-PCR02:57

Real Time RT-PCR

52.0K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
52.0K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

5.9K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
5.9K
Sanger Sequencing01:57

Sanger Sequencing

801.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
801.1K
Next-generation Sequencing03:00

Next-generation Sequencing

88.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.0K
RNA-seq03:21

RNA-seq

9.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.4K
Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Erratum: Impact of pre-existing immunity on safety and biodistribution of a single AAV9 vector intrathecal injection in cynomolgus monkeys.

Molecular therapy. Advances·2026
Same author

Comparison of neonatal systemic and intracerebroventricular AAV9 gene therapy delivery demonstrating improved behavioral and phenotypic outcomes in a mouse model of Niemann-Pick disease, type C1.

PloS one·2026
Same author

Metadata assessment of non-human primate studies of AAV9 uncovers potential tissue specific variation in expression efficiency.

Gene therapy·2026
Same author

Impact of pre-existing immunity on safety and biodistribution of a single AAV9 vector intrathecal injection in cynomolgus monkeys.

Molecular therapy. Methods & clinical development·2025
Same author

Comparative assessment of the transduction efficiency and safety associated with the delivery of AAV9-GFP vector via lumbar puncture to cynomolgus macaques with and without anti-AAV9 pre-existing antibodies.

Molecular therapy. Methods & clinical development·2024
Same author

Prenatal AAV9-GFP administration in fetal lambs results in transduction of female germ cells and maternal exposure to virus.

Molecular therapy. Methods & clinical development·2024
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: May 5, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

9.8K

直接的なRNAシーケンシング

Fatih Ozsolak1, Adam R Platt, Dan R Jones

  • 1Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA. fatihozsolak@gmail.com

Nature
|September 25, 2009
PubMed
まとめ
この要約は機械生成です。

直接RNA配列化は,補完的なDNA (cDNA) 変換をバイパスし,バイアスを軽減し,トランスクリプト分析を改善します. この革新的な方法は,トランスクリプトームの包括的な理解のために,高通量,低コストの直接RNAシーケンシングを可能にします.

さらに関連する動画

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

1.9K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.7K

関連する実験動画

Last Updated: May 5, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

9.8K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

1.9K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.7K

科学分野:

  • ゲノミクスとトランスクリプトミクス
  • 分子生物学は分子生物学である.
  • バイオインフォマティックス

背景:

  • ゲノムとトランスクリプトームの理解は,ヒトの生物学と疾患の研究に不可欠です.
  • 現在のトランスクリプトーム分析は,補完的なDNA (cDNA) 変換に依存し,バイアスを導入し,劣化または低量のRNAの分析を制限します.
  • 既存の方法は,ゲノム全体の転写とRNAの動的状態の包括的な理解を妨げています.

研究 の 目的:

  • 前もってRNAからcDNAへの変換なしに,単一分子RNAの直接配列決定法を開発し,適用する.
  • 劣化したRNAに対するバイアスと適性を含む,現在のトランスクリプトーム分析方法の限界を克服するために.
  • トランスクリプトームの完全な理解のために,高スループット,低コスト,そしてバイアスのない直接RNAシーケンシングを可能にします.

主な方法:

  • RNAをcDNAに変換することなく,単一分子RNAの直接配列を解析を行った.
  • ポリアデニル化 [poly (((A) (((+)) ] サッカロマイセス・セレヴィセアRNAのフェムトモルの量で配列を解析した.
  • RNAは,poly (((dT)) オリゴヌクレオチドでコーティングされた表面を使用してキャプチャされ,天然のpoly (((A)) 尾経由で合成による配列化を開始しました.

主要な成果:

  • cDNA変換なしにRNAを直接配列に成功し,新しいアプローチを示した.
  • トランスクリプト3'-エンドの異質性を観察し,RNA処理の洞察を提供した.
  • 特定されたポリアデニル化小核子RNA,非コーディングRNA集団の知識を拡大.

結論:

  • 直接的なRNAシーケンシングは,高スループットと費用対効果の高いトランスクリプトーム分析への道を提供します.
  • この方法は,cDNA合成に関連するバイアスを最小限に抑え,より正確なトランスクリプト特徴と定量化を可能にします.
  • 生物学的および疾患の研究に不可欠なトランスクリプトームの包括的かつ偏見のない理解の目標を達成します.