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関連する概念動画

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

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関連する実験動画

Updated: Jun 20, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

アロステリックリボジームによって媒介される条件付きRNA干渉.

Deepak Kumar1, Chung-Il An, Yohei Yokobayashi

  • 1University of California, Davis, Department of Biomedical Engineering 451 Health Sciences Drive, Davis, California 95616, USA.

Journal of the American Chemical Society
|October 1, 2009
PubMed
まとめ
この要約は機械生成です。

研究者らは,哺乳類の細胞における精密な遺伝子静止制御のための新しいRNAシステムを開発した. この化学誘導性RNA干渉 (RNAi) 方法は,遺伝子発現を調節するためのモジュラーで適応可能なアプローチを提供します.

さらに関連する動画

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

関連する実験動画

Last Updated: Jun 20, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

科学分野:

  • 分子生物学は分子生物学である.
  • バイオテクノロジー バイオテクノロジー
  • 遺伝子規制 遺伝子規制

背景:

  • 条件付きRNA干渉 (RNAi) は,制御された遺伝子サイレンシングを可能にします.
  • 既存の方法はしばしば複雑な遺伝子改変を必要とし,その応用を制限しています.
  • よりシンプルで化学的に誘導可能なRNAiシステムが必要である.

研究 の 目的:

  • 哺乳類の細胞にRNAiの化学誘導のための新しいRNAアーキテクチャを設計する.
  • 条件付き遺伝子サイレンシングのためのコンパクトでモジュラーなシステムを作成する.
  • 様々な分子信号に反応できるRNAiシステムを可能にする.

主な方法:

  • 薬剤誘導性アロステルリボ酵素とマイクロRNA前駆体アナログを組み合わせた新しいRNA構造を設計した.
  • 哺乳類の細胞におけるRNAi活性化のための化学誘導を用いた.
  • 潜在的適応のためのRNA設計のモジュール性を実証した.

主要な成果:

  • 哺乳類の細胞で化学的に誘導可能なRNAiシステムを開発しました.
  • 新しいRNAアーキテクチャは,コンパクトでモジュール化された設計を提供します.
  • このシステムは,RNAアプタマーを介して様々な分子を感知し,それに反応するために適応可能です.

結論:

  • 新しいRNA設計により,遺伝子サイレンシングを精密に化学的に制御できます.
  • このモジュラーシステムは,多用途の条件付きRNAiアプリケーションの構築を容易にする.
  • このアプローチは,研究および潜在的に治療における遺伝子発現を調節するための有望なツールを提供します.