関連する実験動画
Updated: May 12, 2026
08:22
Assessment and Characterization of Hyaloid Vessels in Mice
Published on: May 15, 2019
内皮細胞におけるノリン,Frizzled-4,LRp5のシグナリングは,網膜の血管化のための遺伝プログラムを制御する
Xin Ye1, Yanshu Wang, Hugh Cahill
1Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Cell
|October 20, 2009
まとめ
ノーリン/フリズレッド-4 (Fz4) 信号伝達は,血管の発達に極めて重要です. 内皮細胞におけるFz4の喪失は血管の成長を阻害し,網膜神経細胞の機能不全と潜在的な中枢神経系血管問題を引き起こします.
科学分野:
- 血管生物学 血管生物学とは
- 神経科学は神経科学である.
- 発達生物学 発達生物学とは
背景:
- 血管の構造と機能は,中枢神経系 (CNS) 疾患において極めて重要です.
- Frizzled-4 (Fz4),Lrp5,またはNorrinの変異は網膜の低血管化を引き起こすが,その根本的なメカニズムは不明である.
研究 の 目的:
- ノーリン/Fz4/Lrp信号が血管の発達を制御するメカニズムを定義する.
- 網膜低血管化疾患の細胞基盤を調査する.
主な方法:
- マウスの遺伝子モデルと細胞培養を用いた.
- 内皮細胞におけるFrizzled-4 (Fz4) 信号伝達の役割を分析した.
- 転写因子Sox17.の機能を研究した.
主要な成果:
- 内皮細胞におけるFz4シグナリングの喪失は,血管の欠陥成長と可逆性のある網膜ニューロン静音化をもたらした.
- 内皮細胞におけるFz4の破壊は,小脳内の血脳障壁を損なう.
- 過剰なFz4シグナル伝達は,胚の血管新生を妨げました.
- ノーリン/Fz4/Lrpシグナル伝達によって上位調節されるSox17は,血管新生プログラムの中心です.
結論:
- 不十分なFrizzledシグナル伝達に関連した網膜低血管化疾患の細胞基礎を確立しました.
- 血管の成長,改造,維持,疾患におけるFrizzledシグナリングのより広範な役割を示唆しています.
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
01:24Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.