PDGF A鎖遺伝子は,哺乳類の神経細胞によって,発達期および成熟期に発現する

H J Yeh¹, K G Ruit, Y X Wang

¹Department of Medicine, Washington University Medical Center, Jewish Hospital, St. Louis, Missouri 63110.

Cell

|January 11, 1991

まとめ

ニューロンは,哺乳類の神経系において,血小板由来成長因子A (PDGF A) を広く発現する. これは,ニューロンが,膠質細胞の発達と増殖を制御する上で重要な役割を果たしていることを示唆している.

科学分野:

神経科学は神経科学である.
発達生物学発達生物学について
細胞生物学細胞生物学

背景:

血小板由来成長因子 (PDGF) は,哺乳類の中央神経系の発達に関与しています.
神経発達におけるPDGF Aの正確な役割と局所化は,さらなる調査を必要としています.

研究の目的:

哺乳類の神経系におけるPDGF A鎖 mRNAおよびタンパク質の局所化および発現パターンを調査する.
PDGF A発現の発達調節と組織特異性を理解する.

主な方法:

PDGFのA鎖mRNAを検出するために,in situハイブリダイゼーションが採用されました.
免疫ペロキシダース染色は,免疫反応性PDGF Aタンパク質を特定するために使用されました.

主要な成果:

PDGF A-chain mRNAとタンパク質の高いレベルは,中枢神経系と周辺神経系における胚性および成年マウスニューロンの両方で発見されました.
PDGF Aの発現は発達的に調節され,組織特異的です.
膠質細胞は,PDGFAに対する信号が変化し,神経細胞と比較して弱かった.

結論:

ニューロンはPDGF Aを広く発現し,既知の神経栄養素因子の分布とは対照的です.
PDGF Aの広範囲にわたるニューロンの発現は,神経細胞が in vivo で膠質細胞の増殖と分化を調節する上で重要な役割を担うことを示唆しています.

さらに関連する動画

10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

04:17

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Published on: March 8, 2024

関連する概念動画

01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...