Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.9K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.9K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.3K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.3K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.4K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.4K
Forced Transdifferentiation01:28

Forced Transdifferentiation

2.5K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
2.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Replication-stress-induced chromatin loops protect fork stability.

Nature·2026
Same author

Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.

Cell·2026
Same author

Focused CRISPR screening to design fit-for-purpose CAR T cell therapies.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

DNA repair drives cisplatin-induced neuronal death.

Cell·2026
Same author

Directing fratricide within T cell products using an anti-uPAR chimeric antigen receptor to drive the production of potent therapeutic cells.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Synonymous editing alters ion channel function, favoring prime editing for retinal disease correction.

International journal of biological sciences·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: Mar 30, 2026

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
09:57

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Published on: December 21, 2016

15.4K

直接的な細胞再プログラミングは,加速を加速させる可能性のあるストキャスティックなプロセスです.

Jacob Hanna1, Krishanu Saha, Bernardo Pando

  • 1The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA. Hanna@wi.mit.edu

Nature
|November 10, 2009
PubMed
まとめ
この要約は機械生成です。

体細胞を誘発性多能幹細胞 (iPS) に再プログラムすることは,継続的なプロセスです. 細胞分裂率とナノグは再プログラム速度に影響を与え,細胞分裂を表遺伝的変化の鍵として強調しています.

さらに関連する動画

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

7.0K
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

20.5K

関連する実験動画

Last Updated: Mar 30, 2026

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
09:57

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Published on: December 21, 2016

15.4K
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

7.0K
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

20.5K

科学分野:

  • 幹細胞生物学 幹細胞生物学とは
  • エピジェネティクス エピジェネティクス
  • 分子生物学は分子生物学である.

背景:

  • ソマティック細胞を誘発性多能幹細胞 (iPS) に直接再プログラムすることは,通常,Oct4,Sox2,Klf4,c-Myc.を過剰に発現させることで達成されます.
  • しかし,体細胞のわずかな部分しか再プログラムに成功しない.

研究 の 目的:

  • 再プログラミングプロセスの基礎となる運動学とメカニズムを調査する.
  • 誘導されたプラリポテンシー誘導の効率と速度に影響を与える要因を特定する.

主な方法:

  • マウスの体細胞における再プログラム因子 (Oct4,Sox2,Klf4,c-Myc) の過剰発現.
  • p53/p21経路を阻害する.
  • リン28とナノグールの過剰発現.
  • 再プログラム運動と細胞増殖率の定量分析.

主要な成果:

  • 再プログラミングは継続的なストカスティックなプロセスであり,ほとんどの細胞は,持続的な発現と成長によって最終的にiPS細胞になります.
  • p53/p21またはLin28過剰発現の抑制は,細胞分裂率の増加に比例して,iPS細胞形成を加速した.
  • ナノゲンの過剰発現は,細胞分裂率とは無関係に再プログラムを加速した.
  • 細胞分裂速度依存型と細胞分裂速度依存型の異なる再プログラム加速度が特定されました.

結論:

  • 細胞分裂の数は,多能性へのエピジェネティック再プログラミングを推進する重要なパラメータです.
  • 細胞増殖率とナノグのような特定の要因の両方が,再プログラム運動を調節する上で重要な役割を果たします.