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関連する概念動画

DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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関連する実験動画

Updated: May 24, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

コヘシンアセチル化により,複製フォークの速度が加速されます.

Marie-Emilie Terret1, Rebecca Sherwood, Sadia Rahman

  • 1Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.

Nature
|November 13, 2009
PubMed
まとめ

複製因子C (RFC) -CTF18クランプロードは,複製フォーク速度とコヘシンアセチル化を調節し,これはゲノム複製とDNA損傷の防止に不可欠です. この研究は,フォークの進行のためのコヘシン修正を含む新しいメカニズムを明らかにしています.

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは

背景:

  • コヘシンは姉妹染色体を結びつけ,染色体のアクセシビリティを調節する.
  • 複製フォークは,DNA複製中にコヘシンに関連した障害を克服する必要があります.
  • レプリケーションフォークがコヘシンをナビゲートするメカニズムは,ほとんど不明です.

研究 の 目的:

  • 複製フォークの進行における RFC-CTF18 クランプ・ローダーの役割を調査する.
  • DNA複製におけるコヘシンアセチル化の機能を解明する.
  • コヘシン調節とゲノム安定性の関連性を理解する.

主な方法:

  • ヒト細胞における単分子分析.
  • コヘシンアセチル化とその複製フォークへの影響に関する研究.
  • コヘシンアセチルトランスファーゼ (ESCO1,ESCO2) とロバーツ症候群患者の細胞に変異がある細胞の分析.

主要な成果:

  • RFC-CTF18は複製フォークの速度,間隔,再起動を制御し,SMC3アセチル化と姉妹染色体凝結に不可欠です.
  • コヘシンアセチル化は,複製フォークのプロセシビティに不可欠であり,その欠如は遅いフォークにつながります.

さらに関連する動画

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome
05:22

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

関連する実験動画

Last Updated: May 24, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome
05:22

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

  • SMC3のアセチル化状態は,WAPLとPDS5Aとのコヘシン相互作用を調節することによってフォークスピードを決定する.

    • 結論:

    • コヘシン・ポスト・トランスレーション・モディフィケーションと構造再構築によるコヘシン・ポスト・トランスレーション・モディフィケーションと構造再構築によるクランプ・ローダー依存のフォーク・プログレッションのための新しいメカニズムが説明されています.
    • このコヘシンアセチル化に依存するプロセスの失調は,DNA損傷の蓄積につながります.
    • この経路の欠陥は,ロバーツ症候群のようなコヘシノパシーに寄与する可能性があります.