bcl-2 P1プロモーターのiモチーフは 8:5:7ループの折りたたみパターンで意外と安定した構造を形成します
Samantha Kendrick1, Yoshitsugu Akiyama, Sidney M Hecht
1Arizona Cancer Center, 1515 North Campbell Avenue, Tucson, Arizona 85724, USA.
Journal of the American Chemical Society
|November 14, 2009
まとめ
bcl-2 プロモーターは
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- bcl-2プロトオンコゲンの転写調節は複雑で,P1プロモーターおよび調節タンパク質が関与しています.
- P1サイトより上流にあるGC豊富な領域は,bcl-2プロモーターの活性にとって極めて重要です.
- このGC豊富な領域は,G-四重複とiモチーフDNA二次構造を形成することができます.
研究 の 目的:
- bcl-2プロモーターのポリピリミジンに富んだ鎖によるi-モチーフDNA二次構造形成を調査する.
- bcl-2 i-モチーフ構造の折り畳みパターンと安定性を特徴付ける.
- タンパク質と小分子認識のためのこの構造の潜在能力を探求する.
主な方法:
- 変異に関する研究.
- 円形二重光 (CD) スペクトロスコピー
- 熱安定性分析は,熱安定性を分析する.
- 化学的な足跡 (ブロミン)
- 光チミンアナログ組み込み測定法
主要な成果:
- ポリピリミジンに富んだ鎖は,6.6.の移行pHを持つ主要な分子内iモチーフ構造を形成する.
- 8:5:7ループ形状は,pH6.1で優位な構造であり,複数の方法によって確認されています.
- トップ・ループの領域でキャピング構造の証拠が観察されました.
- iモチーフ構造は,タンパク質と小分子相互作用の潜在的な場所を示しています.
結論:
- bcl-2プロモーターのGC豊富な領域は,i-モチーフDNA二次構造を形成することができます.
- 非B-DNAへの適合的移行は,bcl-2トランスクリプションを調節する可能性があります.
- i-motifの構造は,治療的ターゲティングのための新しい可能性を提供します.
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