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関連する概念動画

Organization of Genes02:07

Organization of Genes

Overview
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
15:13

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

暗号化されたエクソン.

J M Nigro1, K R Cho, E R Fearon

  • 1Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231.

Cell
|February 8, 1991
PubMed
まとめ
この要約は機械生成です。

研究者らは,スプライシング中に遺伝子エクソンが順番外になっている乱雑なRNAトランスクリプトを発見した. この新しいRNA産物形成は,遺伝子発現とRNA処理の伝統的な理解に挑戦しています.

さらに関連する動画

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

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関連する実験動画

Last Updated: Jun 25, 2026

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
15:13

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • RNA スプライシング

背景:

  • 代替RNAスプライシングは,タンパク質の多様性を生み出すための重要なメカニズムです.
  • スプライシング中のエクソン結合の正確な順序は,機能トランスクリプトの生成に不可欠です.
  • 候補腫瘍抑制遺伝子DCCは,細胞の成長と発達に関与しています.

研究 の 目的:

  • 異常なRNAスプライシングイベントの発生と性質を調査する.
  • 非シーケンス的なエクソン結合から生じる新しいRNA製品を特定する.
  • 正常な細胞と腫瘍細胞における暗号化されたトランスクリプトの影響を理解する.

主な方法:

  • 敏感なRNA発現アッセイは,異常なトランスクリプトを検出するために使用されました.
  • ポリメラーゼ連鎖反応 (PCR) 増幅は,特定のRNAセグメントを分離するために使用されました.
  • クローニングとシーケンシングのテクニックは,異常トランスクリプトの正確な構造を決定するために使用されました.

主要な成果:

  • DCC遺伝子のいくつかの異常にスプライスされたトランスクリプトが特定されました.
  • これらのトランスクリプト内のエクソンは,スプライスサイトで正確に結合されましたが,乱雑な順番で.
  • 4つの異なるタイプのスクランブルトランスクリプトが特徴付けられ,それぞれ異なるエクソンペアが含まれています.
  • 暗号化されたトランスクリプトは,様々な正常細胞および癌細胞,主に非ポリアデニル化サイトプラズミックRNAの低レベルで検出されました.

結論:

  • スプライシング機械は,ゲノムのDNA配列から逸脱して,非連続的な順序でエクソンを結合することができます.
  • このプロセスは,潜在的に機能が変化した新しいRNA製品を生成します.
  • 発見は,遺伝子発現の調節への影響を持つ,以前未知のRNA処理のメカニズムを明らかにしています.