Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.

The Plant cell·2026
Same author

Molecular mimicry of a pathogen virulence target by a plant immune receptor.

Science (New York, N.Y.)·2026
Same author

Haplotype-phased genomes of the barley leaf rust pathogen reveal evidence of repeat element expansion and somatic hybridization.

G3 (Bethesda, Md.)·2026
Same author

Lifestyle-associated variation in type IV secretion systems between phytopathogenic and environmental <i>Ralstonia</i>.

Microbial genomics·2026
Same author

Haplotype-Resolved Genomes of Diverse Oat Crown Rust Isolates Reveal Global Dispersion of Long-Lived Clonal Haplotypes with Limited Recombination.

Molecular plant-microbe interactions : MPMI·2026
Same author

Starch synthesis in Brachypodium distachyon endosperm occurs in dynamic, connected amyloplast compartments.

The New phytologist·2025

関連する実験動画

Updated: Jun 18, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

シンプルな暗号が,TALエフェクタによるDNA認識を制御しています.

Matthew J Moscou1, Adam J Bogdanove

  • 1Department of Plant Pathology and Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA 50011, USA.

Science (New York, N.Y.)
|November 26, 2009
PubMed
まとめ

XanthomonasバクテリアからのTALエフェクターが宿主DNAと結合して病気を引き起こす. TALエフェクタにおける反復変数残留物ペアは,DNA標的特異性を直接決定し,新しい認識機構を明らかにします.

科学分野:

  • 分子生物学は分子生物学である.
  • 植物病理学 植物病理学
  • 遺伝学 遺伝学とは

背景:

  • TALエフェクターは,宿主植物DNAに結合するXanthomonas細菌によって分泌されるタンパク質です.
  • これらのエフェクターは,宿主遺伝子を活性化または抑制することによって,植物病原性において重要な役割を果たします.
  • TALエフェクターDNA結合の特異性は,変数の繰り返しによって決定されるが,認識メカニズムは不明である.

研究 の 目的:

  • TALエフェクターが特定のDNA標的部位を認識し,結合するメカニズムを解明する.
  • TALエフェクター内で変数がどのように繰り返されるかを理解するには,DNA標的特異性を授与します.
  • 研究とバイオテクノロジーにおけるこの理解の潜在的な応用を探求する.

主な方法:

  • TALエフェクタリピート領域とそのDNAターゲットの構造的・機能的な関係に関する分析.
  • DNA結合特異性への影響を評価するために,繰り返し変数残基のサイト指向型変異.
  • DNA-タンパク質の相互作用と機能的影響を確認するために,in vitroおよびin vivoアッセイを行います.

主要な成果:

  • 各TALエフェクタリピート内の特定の残基のペアと標的DNAヌクレオチドの間の直接的な相関が確立されました.

さらに関連する動画

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
11:44

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Published on: September 18, 2014

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

関連する実験動画

Last Updated: Jun 18, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
11:44

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Published on: September 18, 2014

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

  • この残基-ヌクレオチドのペアリングは,周囲のDNA配列の文脈から独立して機能します.
  • 発見は,タンパク質-DNA認識のためのこれまで未知のメカニズムを明らかにします.

    • 結論:

    • この研究では,反復変数残基に基づいて,TALエフェクターDNA結合特異性のシンプルで直接的なコードを特定しています.
    • このメカニズムは,TALエフェクターが正確なDNAターゲティングをどのように達成するかを説明します.
    • この発見は,TALエフェクター標的部位の正確な予測を可能にし,新しいバイオテクノロジーツールの道を開きます.