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Updated: Jun 18, 2026

09:16
Eukaryotic Polyribosome Profile Analysis
Published on: June 15, 2010
モノメア酵母と哺乳類のSec61複合体の構造は,翻訳リボソームと相互作用する
Thomas Becker1, Shashi Bhushan, Alexander Jarasch
1Gene Center Munich and Center for Integrated Protein Science, Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
まとめ
タンパク質伝導チャネル (PCC) Sec61複合体は,タンパク質転位においてオリゴーマーではなくモノーマーとして機能する. 構造データは,単一のSec複合体が,翻訳中の新生タンパク質の動きをどのように促進するかを明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- 細胞生物学 細胞生物学
背景:
- Sec61/SecY複合体は,分泌および膜タンパク質の転位に不可欠なタンパク質伝導チャネル (PCC) として作用する.
- Sec複合体はオリゴーマーを形成できるが,PCCの機能的オリゴーマー状態は議論の余地がある.
研究 の 目的:
- コトランスレーション性タンパク質転位中のエウカリオットSec61複合体のオリゴメール状態と構造的基礎を決定する.
- リボソームとSec複合体の相互作用を,アイドル状態とアクティブ状態の両方で調査する.
主な方法:
- ユカリオットリボソーム-Sec61複合体のサブナノメートル解像度の冷凍電子顕微鏡 (Cryo-EM) 検査.
- 生化学データと構造学的発見の統合.
主要な成果:
- Sec61複合体は,オリゴーマーではなくモノーマーとして,アイドル状態とアクティブ状態の両方で存在します.
- モノメリックなSec複合体は,ユニバーサル結合部位にある2つのサイトプラズマループを通じてリボソームと相互作用する.
- 活性転位の間,新生ポリペプチド鎖はリボソームトンネルと単体PCCの中央孔を占め,Sec61ループ6.6と接触する.
結論:
- 単一のSec61複合体は,コトランスレーション性タンパク質転位に十分である.
- この研究は,膜を横断するタンパク質輸送におけるモノメリックSec複合体の機能の構造的基礎を提供します.
関連する概念動画
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Protein Translocation Machinery on the ER Membrane
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Cotranslational Protein Translocation
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...

