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Updated: Jun 18, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

プレミクロRNA核輸出機械の高解像度構造

Chimari Okada1, Eiki Yamashita, Soo Jae Lee

  • 1Institute for Protein Research, Osaka University, 3-2 Yamada-oka, Suita, Osaka 565-0871, Japan.

Science (New York, N.Y.)
|December 8, 2009
PubMed
まとめ

前駆微RNA (pre-miRNA) の核輸出には,エキスポートイン-5 (Exp-5) とランGTP.が含まれています. この研究は,この複合体の構造を明らかにし,輸出のためのプレミRNAを認識し,保護する方法を示しています.

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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Single-Molecule Imaging of Nuclear Transport12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

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Nuclear Export01:42

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Nuclear Export of mRNA02:31

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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