自己分裂リボ酵素が広く発生している

Chiu-Ho T Webb¹, Nathan J Riccitelli, Dana J Ruminski

¹Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697 USA.

Science (New York, N.Y.)

|December 8, 2009

まとめ

自己分裂RNAの一種である,デルタ型肝炎ウイルス (HDV) のようなリボ酵素は,様々な生物に広く存在しています. これらの豊富なリボ酵素は,重要な,まだ未知の生物学的機能を示唆しています.

科学分野:

分子生物学は分子生物学である.
RNA 生物学 RNA 生物学
ゲノミクスゲノミクスとは

背景:

肝炎デルタウイルス (HDV) と細胞質ポリアデニレーション要素結合タンパク質3 (CPEB3) リボ酵素は自己分裂RNAである.
彼らは,最小限の配列保存と保存された内蔵された二重擬似ノート構造を共有しています.

研究の目的:

HDVのようなリボジームの折りたたみを形成する新しい配列を特定するために.
自然界におけるこれらのリボ酵素の分布と生物学的活動を調査する.

主な方法:

潜在的なリボジーム配列を特定するために,二次構造ベースの計算検索が使用されました.
特定された配列の自己分裂活性を確認するために,in vitroアッセイが使用されました.
シーケンスホモロジーの検索は,様々な分類にわたって発見を拡大するために行われました.

主要な成果:

アクティブなHDVのようなリボジームは,海洋生物,ネマトード,節足類,細菌,昆虫ウイルスで発見されました.
これらのリボ酵素は,植物,真菌,そして単細胞の真核生物でも発見されました.
アノフェレス・ガンビア (Anopheles gambiae) の初期発達の段階で,差異表現と自己割れ活動が観察されました.

結論:

HDVのようなリボ酵素は,自然全体に広く分布しています.
これらの自己分裂RNAの豊富で多様な発現は,多様で重要な生物学的役割を示唆しています.

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