DNAベースのマイクロアレイプラットフォームによる陽性血液培養から細菌種を正確かつ迅速に特定する:観察研究
Päivi Tissari1, Alimuddin Zumla, Eveliina Tarkka
1Division of Clinical Microbiology, Helsinki University Hospital Laboratory, Helsinki, Finland.
Lancet (London, England)
|December 17, 2009
まとめ
マイクロアレイ技術を用いた新しい分子セプシス検査は,非常に敏感で特定の細菌の識別を提供します. この急速な方法は,従来の培養と比較して診断を大幅に早め,早期にセプシス管理を可能にします.
科学分野:
- 分子診断は分子診断です.
- 微生物学 微生物学とは
- 感染症 感染症は感染症です.
背景:
- DNAベースのマイクロアレイプラットフォームは,迅速な病原体検出を提供します.
- 新しい分子性セプシスアッセイのパフォーマンスを評価することは,臨床応用にとって極めて重要です.
研究 の 目的:
- 新しい分子性セプシスアッセイの感度,特異性,回転時間を評価する.
- バクテリアの識別のための従来の培養ベースの方法と比較するために,新しい測定法.
主な方法:
- バクテリアの遺伝子 (gyrB, parE, mecA) を標的とした新しいPCRとマイクロアレイアッセイが使用されました.
- セプシス疑いのある患者からの2107個の陽性血液培養物を分析した.
- 感度,特異性,および回転時間は,CLSIガイドラインに従って計算されました.
主要な成果:
- 検査により,陽性サンプルの86%で病原体が検出されました.
- 臨床感度は94.7%,特異性は98.8%でした.
- 測定は,従来の培養方法よりも18時間早く行われた.
結論:
- マイクロアレイベースの測定は,非常に敏感で特定の細菌の識別を提供します.
- この急速な分子測定法により,早めに,エビデンスに基づいたセプシスの管理が容易になります.
- このアッセイは,臨床性セプシスの治療経路を改善する可能性があることを示しています.
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