アラビドプシス・ポリコンブ (Arabidopsis Polycomb) の標的の長い反意味の文字による冷たい誘発による静音化
Szymon Swiezewski1, Fuquan Liu, Andreas Magusin
1John Innes Centre, Colney, Norwich, NR4 7UH, UK.
Nature
|December 17, 2009
まとめ
アラビドプシス・タリアナ (Arabidopsis thaliana) のFLC遺伝子の冷凍誘導による非コーディングアンチセンセスのトランスクリプトは,表遺伝的サイレンシングを開始する可能性があります. これらのトランスクリプトは,FLCの感覚トランスクリプションを一時的に減少させ,長期記憶のためのポリコンブ機械の採用を先行するようです.
科学分野:
- 植物分子生物学 植物分子生物学
- エピジェネティクス エピジェネティクス
- トランスクリプションに関する規則
背景:
- ユカリオットのトランスクリプションは,主に未知の機能を持つ多数の非タンパク質コーディングRNAを生成します.
- アラビドプシス・タリアナのFLC遺伝子は,花開の時間を調節し,クロマチン経路の標的である.
- 染色体調節におけるノンコーディングRNAの役割を理解することは極めて重要です.
研究 の 目的:
- FLC遺伝子を用いて,非コーディングRNAとクロマチンの調節の関連性を調査する.
- FLCの非コーディングトランスクリプトと,冷凍誘発の表遺伝子静止におけるその役割の特徴を明らかにする.
主な方法:
- FLCの非コーディングトランスクリプトを特徴付けるための偏らない戦略.
- 寒さを含む様々なミュータントと治療における感覚/反感覚のトランスクリプトレベルの分析.
- レポーター遺伝子解析は,FLCアンチセンセスプロモーター配列の機能をテストするために行われます.
主要な成果:
- 感覚と反感覚のFLCトランスクリプトレベルは一般的に相関しているが,冷凍処理された植物では独立して変化する.
- FLCロクスをカバーする長い非コーディングアンチセンセスのトランスクリプトのUPREGULATIONは,冷たい曝露と一致し,他の言語化マーカーに先行します.
- FLCアンチセンセスのトランスクリプトは,FLCセンセスのトランスクリプションを一時的に静止させ,そのプロモーター配列は,レポーター遺伝子に寒さによる静止を与えます.
結論:
- 冷たく誘発されたFLCアンチセンセスのトランスクリプトは,FLCの表遺伝的沈黙の初期に重要な役割を果たし,一時的に感覚トランスクリプションを減少させる作用をします.
- このアンチセンセスのトランスクリプションは,ポリコンブ機械が表遺伝的記憶を授与することで,寒冷感知機構の初期の部分である可能性があります.
- 3'遺伝子末端からのアンチセンセスの転写は,感覚転写の条件依存の調節のための一般的なメカニズムである可能性があります.
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