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関連する概念動画

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
Potential Due to a Polarized Object01:29

Potential Due to a Polarized Object

A neutral atom consists of a positively charged nucleus surrounded by a negatively charged electron cloud. When placed in an external electric field, the external electric force pulls the electrons and nucleus apart, opposite to the intrinsic attraction between the nucleus and the electrons. The opposing forces balance each other with a slight shift between the center of masses of the nucleus and the electron cloud, resulting in a polarized atom. On the other hand, a few molecules, like water,...
Atomic Nuclei: Nuclear Relaxation Processes01:23

Atomic Nuclei: Nuclear Relaxation Processes

In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis,  the precessing magnetic moments are randomly oriented around the z-axis. This...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Group Polarization01:01

Group Polarization

Group polarization is the strengthening of an original group attitude following the discussion of views within a group (Teger & Pruitt, 1967). That is, if a group initially favors a viewpoint, after discussion the group consensus is likely a stronger endorsement of the viewpoint. Conversely, if the group was initially opposed to a viewpoint, group discussion would likely lead to stronger opposition.

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関連する実験動画

Updated: Jun 16, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

ex situ ダイナミックな核極化のための極化行列の最適化

Christian Ludwig1, Ildefonso Marin-Montesinos, Martin G Saunders

  • 1School of Cancer Sciences, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT, UK.

Journal of the American Chemical Society
|February 6, 2010
PubMed
まとめ

ダイナミックな核極化により,NMRスペクトロスコピーの感度が向上する. 最適なマトリックス設計により,極化効率が向上し,薬剤のような分子のための2Dヘテロコーレレントスペクトルの取得が加速されます.

科学分野:

  • 核磁共振 (NMR) スペクトロスコピー
  • ハイパーポラライゼーション技術
  • 化学物理 化学物理

背景:

  • ダイナミック・ニュクレア・ポラライゼーション (DNP) は,低濃度のサンプルを分析する上で極めて重要な,NMRの感受性を大幅に高めます.
  • DNP超極化の効率は,極化行列の組成,特に根幹分子接触とスピン拡散に決定的に依存しています.
  • 偏振マトリックスを最適化することは,NMR実験における信号強化を最大化するための鍵です.

研究 の 目的:

  • ダイナミックな核極化のための最適な極化行列を設計するためのコンセプトを提示する.
  • ハイパーポラライゼーション効率とポラライゼーション移転に対するマトリックス組成の影響を調査する.
  • 2DヘテロコーレレントNMRスペクトルの迅速な取得のための最適化されたマトリックスの使用を実証する.

主な方法:

  • ポラライゼーションマトリックス設計のための新しいコンセプトの開発.
  • 設計されたマトリックスを動的核極化実験に適用する.
  • 薬剤のような小分子のための2Dヘテロコーレレートスペクトルの取得.

主要な成果:

  • 最適化されたマトリックス設計は,ハイパーポラライゼーションプロセスの効率を高めます.

さらに関連する動画

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Hyperpolarized Xenon for NMR and MRI Applications
16:20

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

関連する実験動画

Last Updated: Jun 16, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Hyperpolarized Xenon for NMR and MRI Applications
16:20

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

  • 2Dヘテロコーレレートスペクトルの取得が成功しました.
  • スペクトルは,90分のハイパーポラライゼーション期間を経て,1〜2分以内に迅速に得られた.

    • 結論:

    • 最適な偏極化マトリックス設計は,NMRスペクトロスコーピーの効率的な超偏極化に不可欠です.
    • 提示されたコンセプトは,小分子のための貴重な2D NMRデータの迅速な取得を可能にします.
    • このアプローチは,化学的および生物学的システムにおける構造的および動的研究を加速させるのに有望である.