酵母TFIIDにおける支配的な負の変異は,二重のDNA結合領域を定義する

P Reddy¹, S Hahn

¹Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

Cell

|April 19, 1991

まとめ

酵母TFIIDのC末端領域は,成長と遺伝子活性化に不可欠です. TFIIDの繰り返しにおけるDNA結合に影響する変異は,分割されたDNA結合機能を示唆し,転写因子相互作用に影響を与える.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
バイオケミストリーバイオケミストリー

背景:

酵母TFIID (転写因子II D) のC末端領域は,細胞の成長と転写の調節に不可欠である.
TFIID内の機能ドメインを理解することは,真核生物の遺伝子発現機構の解読の鍵です.

研究の目的:

酵母TFIIDのC端部内の重要な残留物を特定するために,その基本的な機能に責任があります.
TFIIDのC末端内の繰り返し領域がDNA結合と転写活性化における役割を調査する.

主な方法:

酵母TFIID.における支配的な変異を隔離するための遺伝子スクリーニング.
TFIIDのC末端の繰り返し内のアミノ酸置換の分析.
変異TFIIDタンパク質のDNA結合能力と細胞成長フェノタイプの評価.

主要な成果:

5つの支配的なTFIID変異が特定され,すべてDNA結合の喪失を示した.
4つの変異は,C末端のN末端の繰り返しで単一のアミノ酸の置換を含む.
C末端のリピートの変異はまた,DNA結合と細胞成長を損なっており,リピート間のDNA結合機能の分割を示しています.
野生型のTFIIDの過剰発現は,突然変異体の支配的な悪影響を救った.

結論:

酵母TFIIDの保存されたC端180アミノ酸は,重要な成長と転写活性化の機能を備えています.
酵母TFIIDのDNA結合能力は,その2つのC末端66-67アミノ酸リピートに分布しています.
支配的なTFIID変異は,野生型TFIIDの他の重要な転写因子との相互作用を妨害することによって機能する可能性が高い.

さらに関連する動画

09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Published on: March 30, 2015

9.3K

11:27

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Published on: February 7, 2015

20.2K

関連する概念動画

01:28

Yeast Signaling

17.3K

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

Mutations

Incomplete Dominance

Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.

30.0K

02:40

Ligand Binding Sites

15.0K

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...

15.0K

01:23

Negative Regulator Molecules

38.5K

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

38.5K

01:03

Single-Strand DNA Binding Proteins

16.7K

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...