KaiCのATPアゼ活性が上昇すると,Synechococcus elongatusの細胞分裂に昼夜間のチェックポイントが適用されます
Guogang Dong1, Qiong Yang, Qiang Wang
1Center for Biological Clocks Research, Department of Biology, Texas A&M University, College Station, 77843-3258, USA.
Cell
|February 25, 2010
まとめ
シアノバクテリアの昼間時計は,FtsZ環の形成を調節することによって,細胞分裂をゲートします. キークロックタンパク質 KaiC
科学分野:
- シルカディアン生物学
- 細胞サイクル調節 細胞サイクル調節
- 微生物の生理学とは
背景:
- シルカディアンクロックは,生理学や行動における日々のリズムを調整する.
- 細胞循環は,ある種の生物の昼夜時計と結びついている.
- シアノバクテリアの昼間時計が細胞分裂をゲートするメカニズムは不明である.
研究 の 目的:
- Synechococcus elongatus.で細胞分裂の昼間ゲートのメカニズムを調査する.
- サイトキネシスの調節に関与するクロックコンポーネントを特定する.
主な方法:
- ワイルド型シネココックスのタイムラップス顕微鏡.
- KaiCおよびKaiAタンパク質を含む,重要な昼間時計の構成要素の分析.
主要な成果:
- 細胞分裂ゲーティングは,野生型のSynechococcus elongatusで確認されました.
- ATPaseの活性に関連したKaiCタンパク質の特定の状態は,FtsZ環の形成を阻害する.
- KaiAとは独立してKaiCのリン酸化を刺激する新しい活性が発見されました.
結論:
- 昼間の時計は,FtsZ環の形成を阻害することによって,Synechococcus elongatusの細胞分裂をゲートします.
- KaiCのATPアゼ活性とリン酸化は,細胞分裂ゲートと昼夜行動において,異なる役割を果たします.
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