植物ペプチドは,共生における細菌の末端分化を制御する
Willem Van de Velde1, Grigor Zehirov, Agnes Szatmari
1Institut des Sciences du Végétal, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France.
Science (New York, N.Y.)
|February 27, 2010
まとめ
植物から分泌されるノードル固有のシステインに富んだペプチド (NCR) は,豆類の共生における窒素固定リゾビウム菌の末端分化を促進する. これらのペプチドは,抗微生物化合物に似ているが,細菌の細胞運命を制御するために,植物によって再利用される.
科学分野:
- 植物と微生物の相互作用
- 分子生物学は分子生物学である.
- シンビオティックな窒素固定です.
背景:
- 豆類は根の結節を形成し,窒素を固定するRhizobiumバクテリアを宿す.
- Medicago truncatulaのバクテリアのエンドシンビオントは,末端の分化を経験しています.
- この差別化を媒介する植物因子は,以前は知られていなかった.
研究 の 目的:
- Rhizobiumバクテリアの末端分化に起因する植物因子を特定する.
- これらの要因が細菌の細胞運命を左右するメカニズムを解明する.
- 豆類の共生におけるこれらの要因の役割を理解する.
主な方法:
- 潜在的差別化因子としてのノードル固有のシステイン豊富な (NCR) ペプチドを調査した.
- NCR輸送を研究するために,Medicago truncatula dnf1-1シグナルペプチダース変異体を使用しました.
- 他の豆類およびNCRペプチドでチャレンジされた培養リゾビアにおけるNCRsのエクトピック発現を行った.
主要な成果:
- NCRペプチドがライゾビウム菌を標的とし,そこに侵入することを実証した.
- dnf1-1変異体におけるNCR輸送の障害が,細菌の末端分化を防ぐことが示された.
- エクトピックNCR発現と直接ペプチドチャレンジは,細菌における末端分化症状を誘発した.
結論:
- NCRペプチドは,共生細菌の末端分化を引き起こす重要な植物由来因子です.
- 植物は,共生のために細菌の細胞運命を操作するために,先天的な免疫エフェクター (NCR) を再利用します.
- これは,エンドシンビオントに対する宿主制御のための新しい戦略を表しています.
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