アラビドプシスのアピカル・ベース・胚極性の制御は,反抗的な転写因子によって行われます
1Plant Biology Laboratory, The Salk Institute for Biological Studies, University of California San Diego, La Jolla, California 92037, USA.
Nature
|March 2, 2010
まとめ
研究者らは,植物胚の発達を制御する重要な遺伝子を特定した. CLASS III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) 遺伝子は発芽形成を調節し,PLETHORA (PLT) 遺伝子は根の発達を制御し,敵対的な関係を示しています.
科学分野:
- 植物発達生物学 植物発達生物学
- アラビドプシスの胚生成
- 植物における遺伝子調節
背景:
- 植物は,臓器のパターニングのために胚形成の間に頂上基底軸を確立します.
- PLETHORA (PLT) 遺伝子は根の運命を調節するとして知られているが,アピカル/ショット運命を調節する遺伝子は知られていなかった.
- TOPLESS (TPL) は,植物の発達に関与する転写共同抑制剤です.
研究 の 目的:
- アラビドプシスの胚形成におけるアピカル/ショット運命を調節するマスターレギュレータを特定する.
- 芽と根の調節経路の間の遺伝的相互作用を解明する.
- 軸の確立におけるTOPLESS (TPL) の役割を理解するために.
主な方法:
- アラビドプシスのtpl-1変異体を使った遺伝子解析.
- PLTおよびHD-ZIP III遺伝子の遺伝子発現分析.
- 運命決定における遺伝子機能を評価するための誤発現の研究.
主要な成果:
- PLT1およびPLT2遺伝子は,TOPLESS (TPL) の直接標的である.
- CLASS III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) の転写因子は,頂点の運命を支配するメスターレギュレータとして特定されています.
- HD-ZIP III遺伝子は,胚の根の棒を射撃棒に変換することができます.
- ポール特異性において,PLTとHD-ZIP III遺伝子の間には敵対的な関係がある.
結論:
- HD-ZIP III遺伝子はショット運命を授与するのに十分であり,PLT遺伝子は根運命を授与します.
- PLTとHD-ZIP III遺伝子の相互作用は,芽と根の極を確立するために重要です.
- TOPLESS (TPL) は,PLT遺伝子の上流に作用し,基礎/根の運命を調節する.
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