関連する実験動画

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

新しく合成されたヒストンのSV40DNAの複製と好ましい結合

C Crémisi, A Chestier, M Yaniv

|December 1, 1977

まとめ

新しく合成されたヒストンは,SV40ミニクロモソームの複製 in vivoの間に,好ましく複製DNAに組み込まれます. この研究では,パルスラベリング技術を使用して複製中のウイルスDNAとのヒストンの関連性を追跡しています.

科学分野:

分子生物学は分子生物学である.
エピジェネティクスエピジェネティクス
ウイルス学ウイルス学ウイルス学

背景:

ヒストンは,DNAを核細胞に包装する基本的なタンパク質です.
DNA複製中のヒストンの動態を理解することは,ゲノムの完全性を維持するために重要である.
SV40ミニクロモソームは,DNA複製とクロマチンの組立を研究するためのモデルシステムを in vivo で提供しています.

研究の目的:

新しく合成されたヒストンの複製DNAとの好ましい関連性を調査する.
ウイルスのDNA複製中の核細胞組成のプロセスを解明する.

主な方法:

35S-メチオニンで感染した細胞のパルスラベリング.
ゾナル沈殿による成熟し複製するSV40ミニクロモソームの抽出と分離.
ポリアクリラミドゲル電泳 (SDSと酸性尿素) を用いたヒストン含有量の分析.

主要な成果:

新しく合成されたヒストンは,SV40DNAを複製するSV40DNAと好ましく結合することが判明しました.
証拠は,複製中にヒストンの組み込みのための特定のメカニズムを示唆しています.

結論:

新しく合成されたヒストンは,DNA複製中にクロマチンに動的に組み込まれます.
この発見は,ウイルス系におけるクロマチンの組立と複製過程の理解に寄与する.

さらに関連する動画

Primary Culture of Mouse Dopaminergic Neurons

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

関連する概念動画

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...

Primary Culture of Mouse Dopaminergic Neurons

Primary Culture of Mouse Dopaminergic Neurons

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...