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関連する概念動画

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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関連する実験動画

Updated: Jun 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

人間における転写因子結合の変動

Maya Kasowski1, Fabian Grubert, Christopher Heffelfinger

  • 1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.

Science (New York, N.Y.)
|March 20, 2010
PubMed
まとめ

転写因子 (TF) の結合の差異は遺伝子発現に大きく影響し,ヒトの個性や種の多様化に寄与する. これらの変異は,しばしば遺伝的変化と関連しており,進化の過程についての洞察を提供します.

さらに関連する動画

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

関連する実験動画

Last Updated: Jun 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

科学分野:

  • ゲノミクスゲノミクスとは
  • 進化生物学の進化生物学について
  • 分子生物学は分子生物学である.

背景:

  • 遺伝子発現の変異は,種化と表型多様性にとって極めて重要です.
  • 転写因子 (TF) 結合は,遺伝子発現に影響を与える重要な規制メカニズムです.

研究 の 目的:

  • 個体と種間の転写因子結合における全ゲノム規模の差異を調査する.
  • TF結合の変異を遺伝子変化と遺伝子発現の違いと相関させるため.

主な方法:

  • 染色体免疫プレシピテーションに続くシーケンシング (ChIP-seq) を用いて,結合部位をマッピングした.
  • RNAポリメラーゼII (PolII) と核因子kappaB (p65) の結合部位をヒト細胞系で分析した.
  • 人間とチンパンジーのPolII結合パターンを比較した.

主要な成果:

  • ヒト個体間では,PolIIとp65結合部位の有意な差異が観察されました (それぞれ25%と7.5%).
  • TF結合の変異は,単核性多形態と構造的変異と頻繁に関連していました.
  • TF結合の差異はしばしば遺伝子発現の変化と相関しており,機能的関連性を示しています.
  • ヒトとチンパンジーの間で,PolII結合の大きな差異が見られた.

結論:

  • 転写因子結合変異は,個体と種間の違いの主な原因である.
  • これらの発見は,表型多様性と種種の遺伝的基盤についての洞察を提供します.