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Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
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コドン解像度における単一翻訳リボソームのリアルタイムtRNAトランジット

Sotaro Uemura1, Colin Echeverría Aitken, Jonas Korlach

  • 1Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305-5126, USA.

Nature
|April 16, 2010
PubMed
まとめ

この研究は,リアルタイムリボソーム翻訳のダイナミクスを明らかにしています. リボソームは,コドンごとに2つの転送RNA (tRNA) を短期間結合し,転位後,脱酸化tRNAが急速に放出されます.

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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